LOCUS       sequence-160156-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For targeting shRNA construct into human AAVS1 locus, using genome 
            editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under
            U6 promoter, flanked by AAVS1 homology arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Daneshvar K, Pondick JV, Kim BM, Zhou C, York SR, Macklin JA, 
            Abualteen A, Tan B, Sigova AA, Marcho C, Tremblay KD, Mager J, Choi 
            MY, Mullen AC
  TITLE     DIGIT Is a Conserved Long Noncoding RNA that Regulates GSC 
            Expression to Control Definitive Endoderm Differentiation of 
            Embryonic Stem Cells.
  JOURNAL   Cell Rep. 2016 Oct 4;17(2):353-365. doi: 
            10.1016/j.celrep.2016.09.017.
  PUBMED    27705785
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-4- 4"; volume: "17"; issue: "2"; pages: "353-365"
COMMENT     Sequence Label: pAAVS1-shRNA
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    27..863
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 gattacgcca agcttgcatg cctgcaagag cagagccagg aacccctgta gggaaggggc
       61 aggagagcca ggggcatgag atggtggacg aggaaggggg acagggaagc ctgagcgcct
      121 ctcctgggct tgccaaggac tcaaacccag aagcccagag cagggcctta gggaagcggg
      181 accctgctct gggcggagga atatgtccca gatagcactg gggactcttt aaggaaagaa
      241 ggatggagaa agagaaaggg agtagaggcg gccacgacct ggtgaacacc taggacgcac
      301 cattctcaca aagggagttt tccacacgga cacccccctc ctcaccacag ccctgccagg
      361 acggggctgg ctactggcct tatctcacag gtaaaactga cgcacggagg aacaatataa
      421 attggggact agaaaggtga agagccaaag ttagaactca ggaccaactt attctgattt
      481 tgtttttcca aactgcttct cctcttggga agtgtaagga agctgcagca ccaggatcag
      541 tgaaacgcac cagacagccg cgtcagagca gctcaggttc tgggagaggg tagcgcaggg
      601 tggccactga gaaccgggca ggtcacgcat cccccccttc cctcccaccc cctgccaagc
      661 tctccctccc aggatcctct ctggctccat cgtaagcaaa ccttagaggt tctggcaagg
      721 agagagatgg ctccaggaaa tgggggtgtg tcaccagata aggaatctgc ctaacaggag
      781 gtgggggtta gacccaatat caggagacta ggaaggagga ggcctaagga tggggctttt
      841 ctgtcaccaa tcctgtccct agtaaagctt ggtaccccat agagcccacc gcatccccag
      901 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
      961 gaatagaatg acacctactc agac
//
