LOCUS       sequence-160158-        4800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries NEW operon 3 final.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Javidpour P, Deutsch S, Mutalik VK, Hillson NJ, Petzold CJ, Keasling
            JD, Beller HR
  TITLE     Investigation of Proposed Ladderane Biosynthetic Genes from Anammox 
            Bacteria by Heterologous Expression in E. coli.
  JOURNAL   PLoS One. 2016 Mar 14;11(3):e0151087. doi: 
            10.1371/journal.pone.0151087. eCollection 2016.
  PUBMED    26975050
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-14"; volume: "11(3):e0151087. doi"; pages: " 
            10.1371/journal.pone.0151087. eCollection 2016"
COMMENT     Sequence Label: pPJ167(JPUB_006765)
FEATURES             Location/Qualifiers
     source          1..4800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..33)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(150..695)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(230..249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(809..903)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(934..1728)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1045..1064)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1655..1674
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     terminator      4710..4758
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
       61 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
      121 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
      181 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
      241 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
      301 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
      361 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
      421 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
      481 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
      541 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
      601 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
      661 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
      721 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
      781 tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
      841 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      901 gtccaagcga gctctcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
      961 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     1021 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     1081 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     1141 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     1201 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     1261 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     1321 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     1381 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     1441 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     1501 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     1561 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     1621 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     1681 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     1741 tcatcctgtc tcttgatcag atcatgatcc cctgcgccat cagatccttg gcggcaagaa
     1801 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     1861 ttccgaattc atgagatctc gtgctttcta tgagtcgtta aaaagagtat tgacttccct
     1921 atcagtgata gaatacttac agccatgata tcaaactcga gtttaagaag gagatataca
     1981 tatgaagaaa ctggttctga tcaacccaca tccaatgggc aacgtgggcg aagaaaatgt
     2041 gagtgttctg aaccaaatgc cggtgaacct gggctacctg aaagttctga cgccaggtaa
     2101 ttggcaagtg gacattatcg atgagaccca agaatacgcc attgatgaaa atacgggcga
     2161 cattaccttt ggtggtgcgg atctggtggg tattaccagc gtgagttacc aagccgatcg
     2221 cgcctacaag attgcgacgg cgtgtcgtaa acgtggtatt ccggttatta tgggtggcat
     2281 ccatgccacg agtaacccag acgaggccgc gaaatacgtt gatagcgtgg tgattcgtga
     2341 aggcatcacc ctgtggagta ccatcattga cgattttgaa aagggtcgcc tgcgtaagcg
     2401 ttatgatggc ggtctgaccc caatggaaat ttactgtaat ctgagcccag atcgcaagtt
     2461 tctgaaagat aaatacaaat accgttatag cggtattatt acgaccgcgg gttgtccatt
     2521 tgcctgtgaa ttttgtagtg tgccacaatt tcagggtcaa aaatatcgcg agcgtccagt
     2581 ggatgatatt ctgaatgaat ttgaaagcat taaaggtcaa tatcgtggcc tgattctgac
     2641 cgacgaaaac ttttacggtc atagcaaaaa gagtaatgaa cgtgtgcgta ccctgttcaa
     2701 aggcatggtg gaacgcggta tctatcagaa ttggtttggt ttcaccagtc tgaatatcta
     2761 caaagatgac gaaaccctgg aatacatggc gaaaagtggt tgtgtgggtg tgctgattgg
     2821 tattgagagc attgaggaag aaagcctgaa gagtatgaac aaaaacgtga atctgaaaat
     2881 tagtgttgaa aactactatg aggccatcgc gaacattcgt aagcatggtc tggccgtgtg
     2941 gggtacgatg gtttttggta atgatgccga caccagcggt accttcgaac gcgtggccga
     3001 ttttatcctg gatagccata ttgatattat gacctgcggc atcctgtgtc cgtttatcaa
     3061 caccccactg taccaccgcc tgaaccgcga gaatcgtctg tttcgtacgc atttcccaga
     3121 agattggaag tattatacca gtcaccacct gacctacgtg ctgaaaaagc tgacgctgga
     3181 tgaaatgatt gaaggtttcc agtacctgta tgatcgtatc tttgccaccg aagtgctgcg
     3241 caaacgcttc catcgtgcca aagacgaact gaataacaac atgaatgcgg ccatgtttgc
     3301 gttccgtgtt aatctggatt ggcagaatgt gtaccaacat ctgattcaaa acctgaagga
     3361 tctgcatacg agtggcgact atgaagcggc cctgaagtgt tatgtggata ccgacaaaca
     3421 aggtaagggt aaaaaactgg cgagtatcga agccgcccca taaaggaggt tttctaatga
     3481 aaatgaccaa agttgtgatt accggtctgg gtctggtgac cccggtgggt accggcctga
     3541 aaaagagctg ggagagtttc gcccaaggtc gtgatggtgc gtgcgaaatg aagagttttg
     3601 acaccagtct gtacaaagtg catcgtagct gtgaagtgaa agactttttt ctggacgtgg
     3661 agctggagaa tcaaattagt gaaaataccc tgcataaata cgtgtactac gcggcccgcg
     3721 aagccctgct ggaaagtggt ctgcatggtg acaaaaaaag cggtcgcgaa cgttttggca
     3781 tcgccattgg taccctggcg gcggaactga ccccattcga acgtctgctg cgcaaaaata
     3841 cgagcctgaa gaacaacggt ttcgaccata aagtggccgc ggtgtatccg ccaaatagta
     3901 ttacgaaaat gctggccaat tactttaact ttgaaggtca gagtatgatt agtctgaatg
     3961 cctgtagtag cggtaaccat gcgattgcgt gggcctacga tcgcctgatc gataacaagc
     4021 tggatgtggt tatggtgggt ggtgcggata tgattccaca aaccgagttt acccacttcc
     4081 ataatctgaa agcgctgacg ccagacaaat gtcaaccatt tgataagaat cgtaaaggtc
     4141 tgatgattgg tgaaggtgcc ggtatcctga tcatggaacg ttacgaatac gccaaaaacc
     4201 gtggtgccgc gattattgcc gaaatggcgg gctacggtct gagttgcgat ggttttcata
     4261 tgaccgcccc acacccggaa ggtgatggcg ccgtgaaaag tatgagtgat gcgctgaaga
     4321 tggcccgtat gaatccagag gatattggtt acatcaatgc ccatggcacg ggtacgccac
     4381 ataatgatcg taccgagacc attgccatta aacgtgtgtt tggtgagcat gcctacaaaa
     4441 ttccatgtag cagtacgaag agcatgatcg gtcacctgat gggtgcggcg agtgccgtgg
     4501 aaagcgtgat ttgttgcctg gtgctgcgtc atggtatcat tccaccgacc attaactatg
     4561 aaaccccaga tccggagtgc aatctggatg ttaccccgaa taacgcccgt gaagcgaatg
     4621 tgagttgtat tgtgaacaat agttttgcct tcggtggtaa taatgccacc accatttttc
     4681 gcaaactgga ataaaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc
     4741 tttttttttg gagattttca acatgaaaaa attattattg gatcctaaaa gcttcctagg
//
