LOCUS       sequence-160163-        3747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries NEW operon 4 final.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Javidpour P, Deutsch S, Mutalik VK, Hillson NJ, Petzold CJ, Keasling
            JD, Beller HR
  TITLE     Investigation of Proposed Ladderane Biosynthetic Genes from Anammox 
            Bacteria by Heterologous Expression in E. coli.
  JOURNAL   PLoS One. 2016 Mar 14;11(3):e0151087. doi: 
            10.1371/journal.pone.0151087. eCollection 2016.
  PUBMED    26975050
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-14"; volume: "11(3):e0151087. doi"; pages: " 
            10.1371/journal.pone.0151087. eCollection 2016"
COMMENT     Sequence Label: pPJ168(JPUB_006767)
FEATURES             Location/Qualifiers
     source          1..3747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..33)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(150..695)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(230..249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(809..903)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(934..1728)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1045..1064)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1655..1674
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
       61 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
      121 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
      181 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
      241 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
      301 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
      361 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
      421 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
      481 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
      541 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
      601 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
      661 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
      721 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
      781 tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
      841 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      901 gtccaagcga gctctcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
      961 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     1021 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     1081 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     1141 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     1201 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     1261 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     1321 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     1381 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     1441 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     1501 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     1561 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     1621 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     1681 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     1741 tcatcctgtc tcttgatcag atcatgatcc cctgcgccat cagatccttg gcggcaagaa
     1801 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     1861 ttccgaattc atgagatctc gtgctttcta tgagtcgtta aaaagagtat tgacttccct
     1921 atcagtgata gatataatag attcatgata tcaaactcga gtttaagaag gagatataca
     1981 tatggttcgc atctacatgt tcatcggtat cgacattgtg gagattaacc gcattaagaa
     2041 actgtgtatt agtaatgaac gtttcctgaa taagatttac accgtgaagg aactggaata
     2101 ctgtctgcca aaaaagaatc gtcatcaaca tctggccgcg cgttttgcca ccaaagaagc
     2161 catgtttaaa gccctgggta ccggttggac cggtaaaatc aaatggaccg atgtggaact
     2221 gctgaatgat gaaaaaggta aaccatacct gaacttttac ggtaatgtga aagagctggt
     2281 ggaagaaaaa aacattaata ccgccgaagt tagtctgagc cactgcaatg aatacgccat
     2341 tgcccaagtg ctgctggtgc caggtgccgt gagtgccgcc agtagtaaac tgtaaaggag
     2401 gttttctaat gaaaaaacgc gtggttatta ccggtctggg tgccgtgagc ccactgggca
     2461 ataccaagga agaattttgg aatggcctgg ttgagggtaa gagtggcatc gccccaatga
     2521 ccagtctgga tctgagttgt tacaaaagca aactgggtgg cgaagtgcgt gatctgctgc
     2581 cggaaattca tctgggtaaa aaaggtctgc gttacctgaa caaaggcacc tacttcctgg
     2641 gcagtgcggc gaaaatggcc ctggatgata gtaaactgcc acaagatggt agcctggaag
     2701 atacgattgg tatcattatt ggtagcagcc tgggtaattt cagtgaaacc accgattact
     2761 tttacgaaat cattcgcaat aatccaagtg aactgagccc gatgtgcagt tacgacgttg
     2821 ccctgaatag tagcgtgaac tacgtgagtg tgctgtttaa agccaaaggt ccagtgcgta
     2881 ccatcagcgc cggttttacc agtagtattg atgccatcga agatgcctac cgtatgattc
     2941 gtaatgatcg tgccaaagtg atgattacgg gtggtgtgga acaaattagc attgatctgt
     3001 acctgatttt ctatctgcgt aagtatctga gcggtattaa tggtggtcaa gaaattagtg
     3061 ccccatttga tgcgaaacgt aatggtttta tcatggccga aggtagttac gtgattattc
     3121 tggaggaact gcaacatgcg ctggagcgcg gtgcgagtat ttacggtgag attaaaggtt
     3181 ttggtaatac ctacgttggt gccaagaatt acagtctgct ggaacgtgtt agtagtgttg
     3241 agaaagccat gcaagaagcc ctgcaacgtg cgggtatcaa aaaagaagac ctggactgta
     3301 tgagcgttaa tgccaatggt tgtaaaattc aagatccaat cgaagccaac gccattcaat
     3361 ttctgtatgg tgaagatgtg aaacatattc caatcagtgc cattaagagt aacgtgggcg
     3421 agagttacgg tacggccggt gccgcccaac tggtgagtac ggcgatgagt attaacaacg
     3481 gcattattcc acatattatc aaccatcaag gtaaagaacc agaaaataac ctgaatctgg
     3541 tgctgggcaa gccactgcgc aaacaaacca atcatgcgat gatcaacaat atggactttg
     3601 aaggtaacaa tagttgtctg gtggttagtc gtttcattca ataatcggtc agtttcacct
     3661 gatttacgta aaaacccgct tcggcgggtt tttgcttttg gaggggcaga aagatgaatg
     3721 actgtcggat cctaaaagct tcctagg
//
