LOCUS       sequence-160173-       17728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mannitol biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17728)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB705
FEATURES             Location/Qualifiers
     source          1..17728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3289..3305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3289..3305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3302..3324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3313..3329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3337..3343,3344..3361,3362..3367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3382..3403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3531..3549)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            3594..3702
                     /note="incP origin of transfer"
     promoter        3885..3989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3990..4058,4059..4847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITIADNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4208..4227)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(5370..6569)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             6675..7325
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      7814..8517
                     /label=oriV
                     /note="incP origin of replication"
     CDS             9184..10332
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLMHEEIVVQFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10415..11527)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(11706..12689)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(12704..13786)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(13835..14758)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(14770..16212)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 ccggactctg actacaaccg tgcgcaaggc gcattaggga ggatgtatgt atgtaatcgc
       61 ctgcggcatc gttgccggct tggcggctgc ggtggccctg ttgggcttca cgccgatgat
      121 ggaggcgctt gccgccggcg aacgccgcaa ggcactcgcg caatggacgc ggacgatgtt
      181 cctggtgctg ctgcctgtcg tgctgatgtg cgcgcccatc gggtccagca tttacgacgc
      241 cgtgcaagcg gacgctggca agcccatcgc tttccacaac ggccggatca cggtcgtcat
      301 ggccctggtc ggcagcttgg ccgttgtcct ggtcgcggct gcgcgtgcgg tggtcaaccg
      361 caagcatgcc agcttctggt tcgtcggctg ggtgatggcg tcggttttgg ccggcggcgt
      421 cggcgcgatc gccagcgcga agcaactggc gttcctcggc gaacatagcg gcatggtggc
      481 cttcggcttc ttccgcgacc aggtgaagga catgcactgc gatgcggacg tgatcctggc
      541 ccggtgggat gaaaaggcga actcgccggt ggtctaccgc tgcccgaagg cgtacctgct
      601 caacaggttc gcatccgcgc ccttcgtgcc ctggccggac tacaccgagg gggaaagcga
      661 ggatctaggt agggcgctcg cagcggccct gcgggacgcg aaaaggtgag aaaagccggg
      721 cactgcccgg ctttattttt gctgctgcgc gttccaggcc gcccacactc gtttgacctg
      781 gctcgggctg catccgacca gcttggccgt cttggcaatg ctcgatccgc cggagcgaag
      841 cgtgatgatg cggtcgtgca tgccggcgtc acgtttgcgg ccggtgtagc ggccggcggc
      901 cttcgccaac tggacaccct gacgttgacg ctcgcgccga tcctcgtagt cgtcgcgggc
      961 catctgcaag gcgagcttca aaagcatgtc ctggacggat tccagaacga ttttcgccac
     1021 tccgttcgcc tcggcggcca gctccgacag gtccaccacg ccaggcacgg ccagcttggc
     1081 ccctttggcc cggatcgacg caaccaggcg ctcggcctcg gccaacggca agcggctgat
     1141 gcggtcgatc ttctccgcaa cgacgacttc accaggttgc aggtccgcga tcatgcgcag
     1201 cagctcgggc cggtcggcgc gtgcgccgga cgccttctcg cggtagatgc cggcgacgta
     1261 gtacccggcg gcccgcgtgg ccgctacaag gctctcctgg cgttcaagat tctgctcgtc
     1321 cgtactggcg cgcaggtaga tgcgggcgac cttcaacctt cgtccctccg gttgttgctc
     1381 tcgcgtcgcc atttccacgg ctcgacggcg tgcggatcgg accagaggcc gacgcgcttg
     1441 cctcgcgcct cctgttcgag ccgcagcatt tcagggtcgg ccgcgcggcc gtggaagcga
     1501 taggcccacg ccatgccctg gtgaaccatc gcggcgttga cgttgcgcgg ctgcggcggc
     1561 cggctggcca gctccatgtt gacccacacg gtgcccagcg tgcggccgta acggtcggtg
     1621 tccttctcgt cgaccaggac gtgccggcgg aacaccatgc cggccagcgc ctggcgcgca
     1681 cgttcgccga aggcttgccg cttttccggc gcgtcaatgt ccaccaggcg cacgcgcacc
     1741 ggctgcttgt ctaccagcac gtcgatggtg tcgccgtcga tgatgcgcac gacctcgccg
     1801 cgcagctcgg cccatgccgg cgaggcaacg accaggacgg ccagcgcggc agcggcgcgc
     1861 agcatggcgt agcttcggcg cttcatgcgt ggccccattg ctgatgatcg gggtacgcca
     1921 ggtgcagcac tgcatcgaaa ttggccttgc agtagccgtc cagcgccacc cgcgagccga
     1981 acgccggcga aaggtactcg accaggccgg gccggtcgcg gacctcgcgc cccaggacgt
     2041 ggatgcgccg gccgcgtgtg ccgtcgggtc caggcacgaa ggccagcgcc tcgatgttga
     2101 agtcgatgga tagaagttgt cggtagtgct tggccgccct catcgcgtcc cccttggtca
     2161 aattgggtat acccatttgg gcctagtcta gccggcatgg cgcattacag caatacgcaa
     2221 tttaaatgcg cctagcgcat tttcccgacc ttaatgcgcc tcgcgctgta gcctcacgcc
     2281 cacatatgtg ctaatgtggt tacgtgtatt ttatggaggt tatccaatga gccgcctgac
     2341 aatcgacatg acggaccagc agcaccagag cctgaaagcc ctggccgcct tgcagggcaa
     2401 gaccattaag caatacgccc tcgaacgtct gttccccggt gacgctgatg ccgatcaggc
     2461 atggcaggaa ctgaaaacca tgctggggaa ccgcatcaac gatgggcttg ccggcaaggt
     2521 gtccaccaag agcgtcggcg aaattcttga tgaagaactc agcggggatc gcgcttgacg
     2581 gcctacatcc tcacggctga ggccgaagcc gatctacgcg gcatcatccg ctacacgcgc
     2641 cgggagtggg gcgcggcgca ggtgcgccgc tatatcgcta agctggaaca gggcatagcc
     2701 aggcttgccg ccggcgaagg cccgtttaag gacatgagcg aactctttcc cgcgctgcgg
     2761 atggcccgct gcgaacacca ctacgttttt tgcctgccgc gtgcgggcga acccgcgttg
     2821 gtcgtggcga tcctgcatga gcgcatggac ctcatgacgc gacttgccga caggctcaag
     2881 ggctgatttc agccgctaaa aatcgcgcca ctcacaacgt cctgatggcg tacttaccca
     2941 aagaacagct aggagaatca tttatgctca gcacacttcc acaagctcat gcaactttct
     3001 tgaaccgcat ccgcgatgcg gtcgcttccg atgttcgctt ccgcgctctt ctgatcggcg
     3061 gctcttacgt tcacggagga ctcgatgagc actccgattt ggatttcgac atcgttgttg
     3121 aggacaactg ctacgcagat gtcttgtcta cacgcaagga ttttgccgag gcactgcccg
     3181 gcttcctcaa cgcgttcacc ggcgaacatg taggagaacc gcgccttctg atctgcctat
     3241 atggtccgcc actgctacac atcgggggat cagcttggcg taatcatggt catagctgtt
     3301 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3361 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3421 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3481 ggggagaggc ggtttgcgta ttgggcgaat taattcttga agacgaaagg gcctcgtgat
     3541 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagtcga atacggccag
     3601 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
     3661 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgccc ggctgacgcc
     3721 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
     3781 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatagcagcg
     3841 gaaagcagtc gaccacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3901 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3961 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4021 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4081 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4141 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4201 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     4261 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4321 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatc gctgataaca
     4381 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4441 acaacatggg ggatcatgta acacgccttg atcgttggga accggagctg aatgaagcca
     4501 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4561 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4621 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4681 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccggatg
     4741 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4801 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattgggta actgtcagac
     4861 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4921 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4981 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5041 cgggggatca ggaccgctcc cggagcgcaa cccactcact acagcagagc catgtagaca
     5101 acatcccctc cccctttcca ccgcgtcaga gccccgtagc gccccctacg ggctttttca
     5161 tgccctgccc tagcgtccaa gcctcacgcc gcgctcggcc tctctggcgg ccttctggcg
     5221 ctcctgctgc ggcgtccgct cgtgggccgc ggcgggtccg cgcgccggcc tcgtgcgctg
     5281 gcgctcgcgg gcgaggtcca aggcggccgt cttcacgttc tgccttgcgc agatgagata
     5341 gatccgtcaa ttccctatcg tttccacgat cagcgatcgg ctcgttgccc tgcgccgctc
     5401 caaagcccgc gacgcagcgc cggcaggcag agcaagtaga gggcagcgcc tgcaatccat
     5461 gcccacccgt tccacgttgt tatagaagcc gcatagatcg ccgtgaagag gaggggtccg
     5521 acgatcgagg tcaggctggt gagcgccgcc agtgagcctt gcagctgccc ctgacgttcc
     5581 tcatccacct gcctggacaa cattgcttgc agcgccggca ttccgatgcc acccgaagca
     5641 agcaggacca tgatcgggaa cgccatccat ccccgtgtcg cgaaggcaag caggatgtag
     5701 cctgtgccgt cggcaatcat tccgagcatg agtgcccgcc tttcgccgag ccgggcggct
     5761 acagggccgg tgatcattgc ctgggcgagt gaatgcagaa tgccaaatgc ggcaagcgaa
     5821 atgccgatcg tggtcgcgtc ccagtgaaag cgatcctcgc cgaaaatgac ccaaagcgcg
     5881 gccggcacct gtccgacaag ttgcatgatg aagaagaccg ccatcagggc ggcgacgacg
     5941 gtcatgcccc gggcccaccg gaacgaagcg agcgggttga gagcctcccg gcgtaacggc
     6001 cggcgttcgc ctttgtgcga ctccggcaaa aggaaacagc ccgtcaggaa attgaggccg
     6061 ttcaaggctg ccgcggcgaa gaacggagcg tggggggaga aaccgcccat cagcccaccg
     6121 agcacaggtc ccgcgaccat cccgaacccg aaacaggcgc tcatgaagcc gaagtgccgc
     6181 gcgcgctcat cgccatcagt gatatcggca atataagcgc cggctaccgc cccagtcgcc
     6241 ccggtgatgc cggccacgat ccgcccgata tagagaaccc aaaggaaagg cgctgtcgcc
     6301 atgatggcgt agtcgacagt ggcgccggcc agcgagacga gcaagattgg ccgccgcccg
     6361 aaacgatccg acagcgcgcc cagcacaggt gcgcaggcaa attgcaccaa cgcatacagc
     6421 gccagcagaa tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg
     6481 cccggcagca ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga
     6541 attacgatca ggggtatgtt gggtttcacg tctggcctcc ggaccagcct ccgctggtcc
     6601 gattgaacgc gcggattctt tatcactgat aagttggtgg acatattatg tttatcagtg
     6661 ataaagtgtc aagcatgaca aagttgcagc cgaatacagt gatccgtgcc gccctggacc
     6721 tgttgaacga ggtcggcgta gacggtctga cgacacgcaa actggcggaa cggttggggg
     6781 ttcagcagcc ggcgctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg
     6841 ccgaagccat gctggcggag aatcatacgc attcggtgcc gagagccgac gacgactggc
     6901 gctcatttct gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc taccgcgatg
     6961 gcgcgcgcat ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc
     7021 agcttcgctt cctctgcgag gcgggttttt cggccgggga cgccgtcaat gcgctgatga
     7081 caatcagcta cttcactgtt ggggccgtgc ttgaggagca ggccggcgac agcgatgccg
     7141 gcgagcgcgg cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag
     7201 acgccttcga cgaagccggt ccggacgcag cgttcgagca gggactcgcg gtgattgtcg
     7261 atggattggc gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga aagggtgacg
     7321 attgatcagg accgctgccg gagcgcaacc cactcactac agcagagcca tgtagacaac
     7381 atcccctccc cctttccacc gcgtcagacg cccgtagcag cccgctacgg gctttttcat
     7441 gccctgccct agcgtccaag cctcacggcc gcgctcggcc tctctggcgg ccttctggcg
     7501 ctcctgctgc ggcgtccgct cgtgggccgt ggcgcgggtc cgcgcgccgg cctcgtgcgc
     7561 ctggcgctcg cgggcgaggt ccagggcggc cgtcttcacg ttctgccttg cgcagatgag
     7621 atagatcgaa ttgacggatc tagcgtggac tcaaggctct cgcgaatggc tcgcgttgga
     7681 aactttcatt gacacttgag gggcaccgca gggaaattct cgtccttgcg cagaaccggc
     7741 tatgtcgtgc tgcgactcga ngcctgcgcc cttggcttgt ctcgcccctc tccgcgtcgc
     7801 tacggggctt ccagcgcctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg
     7861 gcggccgtct atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac
     7921 cgcggccgcc ggcgttgtgg ataccacgcg gaaaacttgg ccctcactga cagatgaggg
     7981 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
     8041 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
     8101 ttttacgcga gtttcccaga catgatgtgg acaagcctgg ggataagtgc cctgcggtat
     8161 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     8221 cagagtgatg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     8281 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     8341 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctggcgcgt
     8401 gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggct aacgcgggcc
     8461 tcccatcccc ccggctgcgc ccttcggccg cgaacggcct caccccaaaa atggcagcgc
     8521 tggcagtcct tgccattgcc gggatcgggg cagtaacggg atgggcgatc agcccgagcg
     8581 cgacgcccgg aagcattgac gtgccgcagg tgctggcatc gacattcagc gaccaggtgc
     8641 cgggcagtga gggcggcggc ctgggtggcg gcctgccctt cacttcggcc gtcggggcat
     8701 tcacggactt catggcgggg cgggcaattt ttaccttggg cattcttggc atagtggtcg
     8761 cgggtgccgt gctcgtgttc gggggtgaat taattccccg gatcgatccg tcagcttcac
     8821 gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca
     8881 gtccgcagaa acggtgctga ccccggatga atgtcacgta ctgggctatc tggacaaggg
     8941 aaaacgcaag cgcaaacaca aacgacctag cttgcagtgg gcttacatgg cgatagctag
     9001 actgggcggt tttatggcca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     9061 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     9121 gcaggggatc aagatcgacg gatcgatccg gggaattccg gggcaatccc gcaaggaggg
     9181 tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt
     9241 tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc
     9301 ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg
     9361 caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc
     9421 gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg
     9481 accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag
     9541 gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat
     9601 attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg
     9661 ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac
     9721 gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa
     9781 ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc
     9841 ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg
     9901 aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc
     9961 gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag
    10021 aaaacgtccc gttgccaggt cctgatgcac gaggaaatcg tcgtgcagtt tgctggcgac
    10081 cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg
    10141 ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc
    10201 atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc
    10261 gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat
    10321 tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc cacctgcagg
    10381 catgcaagct tcggccgcct aggccgcggc cgcatcaact atcaaacgct tcggttaagc
    10441 ttaaagcaca ccctttctgc gtcctcgtat tgacgcgacg taaaatttca acgagcacgc
    10501 cgggatactt accatattct ctgctaatta tcccgacatc atcggtaaca ataaatgctg
    10561 gataactggt tgctgacgca tccatataac tcatcaaccc cggcgttcca tcaggtacag
    10621 gtttcaacgt ttcaggatca agcgctcgcg catataccca cggcggaaca tgtttacgct
    10681 gcatttcatc ctcaaagaaa caagtgttga gttcaacttg attaaatata tctcggatct
    10741 gactaatatc actgagattg aaagtatcaa ataaaagatg attgaaatca tcacgtttca
    10801 gagattcttt ttcgtaactt ttccagccgc ctccggttat gatataaagg cttttatctc
    10861 cagaaaatga gattttttta tctttcatat aatggcagag taaataaata aagtatggcg
    10921 aaccaataag acaaagatct ttcccttgat tttttattcg ttcaagacta ttcaatgttt
    10981 taacaaaatc tattcgttct tctgttacgg taaatgtcgt aggatataac aattccacca
    11041 aactcataac atatttaaac caaatattat gagcattaaa tctatctggt cccaaattga
    11101 ctaattctat ttgatgatca aaccaactac caacatattt catgccataa ctcacagagc
    11161 ctaagagtct ctcaatactt aatctgtcac gcgccacctg actttttaaa ccattcgtgc
    11221 cgctactggt aaaccaactt tcaatctcgt tttcctgaga agttaataag cgagtaaact
    11281 taaaaaccga tgttgggaat acaggtatgt catcaatttc cgtaatattg tcatctactt
    11341 tgtgtgcctg acagtagtga cgatattctc gacaatgttt ataatgatta cgaaatgcat
    11401 caagcacaag tttctttctg attttttcct gctcgtcgta agaccacact aatggatcgc
    11461 tcgaaaaaat caaatcatca atttctgagc ttgctgtaat ttcttgttta tcaacatatg
    11521 aagtcatacc tgttttactc ctcaagataa tattagaaag tatggcagca ctgctgtcat
    11581 actcttttat acccttcatc tttcaagctg ctgctttgtt ggctgctttc actcacccca
    11641 gtcacatagt tatctatgct cctggggatt cgttcacttg ccgcctcgct gcaactcgaa
    11701 atctattagg tatattccat gtggtacttc ttaatattat catcaacaat attgattaca
    11761 tttttttggc tcatcaaatc attcattggt tcaaaggaca gcaatacact tttcgcacca
    11821 cacttttcaa ttgccaactt agccgcagtt atacactccg tataatttcc gacagcgttt
    11881 tctgcaatta tttcttcaag tttattttcg aaattttcat tagggtgcat ttcaagaaca
    11941 taatcactaa taaatgcacg cgtctcttgt ttagctttat tactatcttc gttatagtta
    12001 actaatatca ttaactgatg gtctatctct gataggtcaa cgtcatattt atccgcaacg
    12061 gctttatatc tttcagcata ttcatatcta acatcattag aatcatccca cttaaagatg
    12121 agaggaatac cttttttggc cgcccactca acaatatgat gactggttgc tgttacatat
    12181 ttccgaggtc cgcctggcgt ataagcatgg ggatttacag atattttagg gaagctataa
    12241 aaatcgttat ctggattaca atagcctgtt gttaaagcat cgttaatgat ttcataacac
    12301 tcttcaaata gttgctgttg atattcaacc gggcgattaa aaaaatgcat ttcatctttt
    12361 ttttcgcaat cactaaaccc taaaataaat ctcccttcac ttaactgatc caataagcaa
    12421 gcttcctccg ctatggcgac aggatgatga gttgtaatga tgtgatttaa tgaaccaatt
    12481 ttaattttct ctgttaaacc gagcagaaaa ccagaaacag tcagaggagc gccgacaaca
    12541 ccattatctg aaaaatgatt ttcatacact aaaatctgtt caaaattcaa cttatcaaca
    12601 tactccgtta tttcctgcat gcgaactata ctttgttctt gaacagttgt tgaattgatg
    12661 aagttaagga agaacaatcc aaatttcatt tctttctcct tagctaatat aatagcgaac
    12721 gttgtttttc tttaagaaat ggcatgacat cagactggaa gagcttcatg gaagcaataa
    12781 tttcgtctac tgttccatta gcttcaaatc cacaacaaat atttgatatt cctgtagcat
    12841 caatgtcttt ttgaattatg tcaatacatt cctgcggcgt tcccacggga ttgatttcgt
    12901 aactgtaatc aatacggcga ttagtatctt tatgtccttt taatacaaag tcacgccact
    12961 gccctttatt gaaatcataa cctcttgttt ggtctgaatc atcaaaaata gtcgtagcat
    13021 tcacataaga atcataccaa tgccccagaa atttccggca aatctctttc gctttaattg
    13081 agtcatgatc tacagatgtt atatatgata agcaatggtc gatattatga atatcgtgcc
    13141 catattcttg agccacttca ttataaagct caagttgtgc tttcttttcg ttagtattta
    13201 taatccaact taatatcatc ggtaggccaa attgagcagc ccactcagtc gtcgaagctg
    13261 attcagccac cacataaacc ggtgcgccac ctctgctata cgccgcgggg tttactttta
    13321 ccttatggaa cttgatatgt tcattatcag cttccatata tccctctgtc atgccattct
    13381 ttatcagccc gtaccagcat tccgctaagg cgcgactgtt attcatatct gtgccgaata
    13441 cgcgaaagtc cttgttgtaa agccctcggc aaataccaaa ccgaaatcgt ccttttgaca
    13501 tttgatccaa taaattcaca tcttcaagtt ggcgtactgg atgggctgtg ggaagaacaa
    13561 tagcggcagt tcctacattc aattttttag tcgcgccaag taaatatgca gcagcgacat
    13621 aagggttacc aagcaaacca aactccgtga aatgatgctc cagtaaccat acggtatcaa
    13681 aaccacactc ctcagagatg cgacctaatt taaccaaacg tttcattacc tctgtttgag
    13741 aaaattgggg aggttggtat gtaagcaaaa agtttccaaa tttcatagag agtccttata
    13801 ttgctatttg agtgatagaa tatctcaata gattttaaga cagagaaatt gcttgatttt
    13861 caatctcaat tctcattcgg cgttcattga ctgtcgcaat agttaaatgt tcaaatgacg
    13921 gttcagtaat atcaacatca atatccagat gatcattatc catcgcgata gcggctttcg
    13981 taaccgattg ataaaaattg cgcaggacca ctaaattttc actcaagtca tgcgaacttc
    14041 ctaacaaaga atatatcttg catcgattac tacgaatatt tgataacaat gtgataactt
    14101 catcttgctt gacccaatta tcgttatttg cagtaaaagc aataaacggt atatcaagat
    14161 acatcatgtt attaattgta gaagctaaat cttcccaacc aaaatcaaga caatctctcg
    14221 caaagacttc agcacccaat ttatggcctt caaaatctag attatccggc aattcattaa
    14281 tgggtagact gagataatca aaccctaaag ctctttcaag agaatatctt aagttaacaa
    14341 caccgactgc ggtgattaaa aacgaagcat tgatttcaga taggcttgca taagctatcc
    14401 gcgcagataa gcttgaagcc aacataccga agttatttat ttttcgtgta gttaaccaat
    14461 caaccactgc taacaagctc tgctttccta tagacattgt aaattcatca attgtccctg
    14521 aactcaatcc aacgtggtga agcgaatcat agcggatcac atgaaatcca ttccgcgata
    14581 aatattccgc cagaccagca aaatgatcca tcctgcgggc aaaaccagac gcaataataa
    14641 tggcattctt tctctttggg ctgttttctt ctggcagcgt ttcccaaaca tgaatttttt
    14701 tatttccttc aacacaaata acgtggtcga tggttttata ttttgattca ttttccatac
    14761 ttttacctat tatgggacaa atacaaggaa cttatcttct tccaggaatc gagtctgttc
    14821 tatttcaacc gcaacatcct tagccgtata gttagatggc ctttcatgag aaatatatgt
    14881 cactaatcgt tgcaacggtc tcattccgtc atgagatcca ccaactcgaa atatgttatt
    14941 cattcctgct tctacaatcc tttccgcacc ttttaatgct aacgcatctc gatatttaaa
    15001 tgatgactcc caaggaaaaa tagatatggt ttgcgtctta tttttttgaa cataaggcaa
    15061 tatttgctca atattatcga cgtgatgaag gtacacacat ctgccaagtg gttgattaaa
    15121 ttccacacct gcatttgact caataatcat ccaacgttga tgaatatcca cctctacttt
    15181 taatccagca aacaagcttt ctttttgaac taaagaatag gccgcctttt catcaaaatc
    15241 ttttttggca ttcggtaata tatgcgcata tagattaagt ttttctatca acgctaactt
    15301 aaattcctca taatgatttc ccatgtaata tatgttttgg gcagaaaaac aagctcgctg
    15361 atcgtaaaaa caaacatcat gagccgcacc tgtcgctgcg gacgtcaaat caacaggatt
    15421 atcgataatg caaagactct ttttagaacc aaatttaatc acatcagcat aagatggcgc
    15481 atgctctacc gcccaattaa tcgcatctgg ccctccccaa gcgacaataa catccgcatg
    15541 tcgcataatt tcttttgcga gtgatgtatc accttggtgg ggccaatata taacagataa
    15601 agagcgcgtt atcggatgat tagggtctac atcaataaaa cttaacgcta atgcattagc
    15661 ggtaaaagga tcggttgacg atgtttttat aatacactga ttcttagtta aaattgcgcg
    15721 taatatagac atgatcccag ataatggaac attacctgcc aacagatgta cagatttacc
    15781 tttcggaaaa gcccgaacat aactttcatc ctgaggtagc cattcatcca tgatatggcg
    15841 agaaccaagt tcattttcta caacatcata aaggccgcct ttagaacata aaatcataga
    15901 tatccaattg gcctctagct tagccatttc ttctgaatat cccatatatt tttttaagtc
    15961 acgaatgtat gtcctgcgtc ttgagtattc ttcatttttc catctttgcc ctaccgtata
    16021 gagaaaattg acaatgttat gcaaccgtaa ttcgttattt ccattacaat caataatgtt
    16081 ttttacatga gagtcattca atattggcag gtaaacacta ttatcaccaa aattaatgga
    16141 ttgcactaaa tcatcacctt cgggaaagat ttcaacctgg ccgttaataa tgaatgaaat
    16201 ttttttagtc atacgtatcc tccaagcctg aattcgttta aacggatcct acgttctctt
    16261 ccagggtgac ccattcaagg tcgatgccgg gattcttcgc cttgaaatca tccgtcagct
    16321 tctgcatccg gatcatgtcg ccgttgttca cggtcgcaat cgtcagcgtt tcggccgaag
    16381 ccataccggc aaacgccagt gctgagcagg cgcccagcag aaaagttctc aatgtcataa
    16441 tcttcctccc agaagatggg gtgtgagcat tcgcttcgct catgagcaat tactcaacaa
    16501 acaacaaaga atgtcaatcg acaatcgttg ctgcgatgcc gaaggacatg gtcatctcgt
    16561 tgatttaatt agaatatttt ttgctcaatt cctgagcaaa aactcagcag tctcttcatc
    16621 agtgatcaga gcattgattt gatggccgac aacggcagcc ctgatcgcct tgaatttacg
    16681 cttgcccttg gccagcccaa tgaccatcga tgcgtcgcgg gacgggatcg gcgccgacgc
    16741 gacgcgctcg ttgatcgggt tgtcgatcaa cctgccgtcg acatcgaaga tccagccgca
    16801 gatctcgccg acagcacctc cgcgcatcag ctccatcatc tcgtccttct ccagaaaacc
    16861 gtcgacgcag agcggcccat cgatgccgag ctcgccgata ccgacgaagg tgacatcggc
    16921 ttgcgcgctc atgttaagcg tcgagcgcac cagctgttgg ccatgcagca gctcccgctc
    16981 ctcggccgag gtgacgagca ccggcagcgg catcggatag tgtcgcgcct tcacggcatc
    17041 ggccatgctg aaaatgacgt tgtaataggc cgccgatccg tccggggcga tattgccggt
    17101 cagcgaaacg atgcggtgat tgggacattc gatcgccggc agctggtcga cggccgcctt
    17161 cagcgtgcgg ccggtaccga tggcaagcac gatcggctcg gcccgtttca gccagcgctc
    17221 gatctcggcc gcggctgcct cggcgatacc gaccgtcgtc gacgacgacg cgggatcgct
    17281 cggcaccacc tccacatgtt tcagcccgaa tttccgccgt aattgattgc cgagctccag
    17341 gcaggcggcg atcggatgat cgagccgcac cttgatcaga cgctcggcga ccgccagcga
    17401 caccagccgc tgcgccgatt gccgcgagat gcccatcgca gcggcgattt catcctgcgt
    17461 acgcccggcg acgtaataga gccagccggc acgcgccgca tcgtcgagcc gtgcaggggt
    17521 ctcggatctt tttaccatct attgcctacc gcgcaaaatg agccggcgtg ccgccgccga
    17581 acatgatttg agtctgtatc gcgccagatc aaaacaaaat tccggcaaat gtcgagcctg
    17641 ttgcaaagag gtcggccaaa taacgccatc actcaagcaa actccgttta ggcaaaccat
    17701 tgcacgacga gatagatctc gagggtac
//
