LOCUS       sequence-160178-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  conditional expression of an shRNA targeting murine Rheb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A
  TITLE     mTORC1 targets the translational repressor 4E-BP2, but not S6 kinase
            1/2, to regulate neural stem cell self-renewal in vivo.
  JOURNAL   Cell Rep. 2013 Oct 31;5(2):433-44. doi: 
            10.1016/j.celrep.2013.09.017. Epub 2013 Oct 17.
  PUBMED    24139800
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.09.017"; journalName: "Cell Rep"; date: 
            "2013-10-31- 31"; volume: "5"; issue: "2"; pages: "433-44"
COMMENT     Sequence Label: pSico-shRheb
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(27..60)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     129..145
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    220..808
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(273..293)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(691..702)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(811..827)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggacgagctg tacaagtagc ggccgcataa cttcgtatag tataaattat acgaagttat
       61 aagccttgtt tgcccgtcat ccttgaagat aaactcgagt ttatcttcaa ggatgacggg
      121 ttttttctcg aggtcgacgg tatcgataag ctcgcttcac gagatcatgt ttaagggttc
      181 cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
      241 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      301 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
      361 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
      421 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
      481 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
      541 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
      601 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
      661 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
      721 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
      781 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgatc gagacctaga
      841 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
      901 agaagcacaa gaggaggagg aggtgggttt tccagtc
//
