LOCUS       sequence-160186-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses an shRNA targeting murine 4E-BP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A
  TITLE     mTORC1 targets the translational repressor 4E-BP2, but not S6 kinase
            1/2, to regulate neural stem cell self-renewal in vivo.
  JOURNAL   Cell Rep. 2013 Oct 31;5(2):433-44. doi: 
            10.1016/j.celrep.2013.09.017. Epub 2013 Oct 17.
  PUBMED    24139800
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.09.017"; journalName: "Cell Rep"; date: 
            "2013-10-31- 31"; volume: "5"; issue: "2"; pages: "433-44"
COMMENT     Sequence Label: pSicoR-sh4EBP2
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(38..71)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    127..715
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(180..200)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(598..609)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(718..734)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(719..735)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gacgagctgt acaagtagga attcgtcgag ggacctaata acttcgtata gcatacatta
       61 tacgaagtta tacatgttta agggttccgg ttccactagg tacaattcga tatcaagctt
      121 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
      181 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
      241 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
      301 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
      361 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
      421 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
      481 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
      541 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
      601 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
      661 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
      721 taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta gcaatacagc
      781 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggtt
//
