LOCUS       sequence-160195-        5836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of dominant negative IkB alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Kim M, Lu F, Zhang Y
  TITLE     Loss of HDAC-Mediated Repression and Gain of NF-kappaB Activation 
            Underlie Cytokine Induction in ARID1A- and PIK3CA-Mutation-Driven 
            Ovarian Cancer.
  JOURNAL   Cell Rep. 2016 Sep 27;17(1):275-288. doi: 
            10.1016/j.celrep.2016.09.003.
  PUBMED    27681437
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-09-27- 27"; volume: "17"; issue: "1"; pages: "275-288"
COMMENT     Sequence Label: pBABE Zeo IkB SR
FEATURES             Location/Qualifiers
     source          1..5836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1318..1334
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2350..2370)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2355..2684
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2535..2670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2694..3068
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             3117..3710
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3911..3928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4082..4670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4162..4181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4848..5639,5640..5708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5471..5490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5709..5813)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc gccaccatgt tccaggcggc
     1381 cgagcgtccc caggagtggg ccatggaggg cccccgcgac gggctgaaga aggagcggct
     1441 actggacgac cgccacgacg ccggcctgga cgccatgaaa gacgaggagt acgagcagat
     1501 ggtcaaggag ctgcaggaga tccgcctcga gccgcaggag gtgccgcgcg gctcggagcc
     1561 ctggaagcag cagctcaccg aggacgggga ctcgttcctg cacttggcca tcatccatga
     1621 agaaaaggca ctgaccatgg aagtgatccg ccaggtgaag ggagacctgg ccttcctcaa
     1681 cttccagaac aacctgcagc agactccact ccacttggct gtgatcacca accagccaga
     1741 aattgctgag gcacttctgg gagctggctg tgatcctgag ctccgagact ttcgaggaaa
     1801 taccccccta caccttgcct gtgagcaggg ctgcctggcc agcgtgggag tcctgactca
     1861 gtcctgcacc accccgcacc tccactccat cctgaaggct accaactaca atggccacac
     1921 gtgtctacac ttagcctcta tccatggcta cctgggcatc gtggagcttt tggtgtcctt
     1981 gggtgctgat gtcaatgctc aggagccctg taatggccgg actgcccttc acctcgcagt
     2041 ggacctgcaa aatcctgacc tggtgtcact cctgttgaag tgtggggctg atgtcaacag
     2101 agttacctac cagggctatt ctccctacca gctcacctgg ggccgcccaa gcacccggat
     2161 acagcagcag ctgggccagc tgacactaga aaaccttcag atgctgccag agagtgagga
     2221 tgaggagagc tatgacacag agtcagagtt cacggagttc acagaggacg agctgcccta
     2281 tgatgactgt gtgtttggag gccagcgtct gacgttatga gaattcgcca gcacagtggt
     2341 cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     2401 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2461 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     2521 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2581 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2641 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt accatggcca
     2701 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     2761 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     2821 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     2881 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     2941 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     3001 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     3061 aggactgacc gatcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     3121 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
     3181 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct
     3241 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     3301 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     3361 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag
     3421 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     3481 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     3541 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     3601 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     3661 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tccgacttgt
     3721 ggtctcgctg ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
     3781 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg
     3841 ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggcgct
     3901 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3961 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4021 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4081 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4141 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4201 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4261 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4321 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4381 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4441 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4501 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     4561 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4621 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4681 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4741 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttgc
     4801 ggccgcaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4861 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4921 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4981 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5041 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5101 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5161 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5221 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5281 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5341 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5401 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5461 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5521 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5581 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5641 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5701 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5761 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5821 ccccgaaaag tgccac
//
