LOCUS       sequence-160197-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfCherry2(11) tagged H2B in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pEGFP_sfCherry2(11)_H2B
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             133..510
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     polyA_signal    640..761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(677..696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     731..750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc aatgtacacc
       61 atcgtggagc agtacgagag agccgaggcc agacacagca ccgtccggct cagatctgga
      121 ggctccggag gcccagagcc agcgaagtct gctcccgccc cgaaaaaggg ctccaagaag
      181 gcggtgacta aggcgcagaa gaaaggcggc aagaagcgca agcgcagccg caaggagagc
      241 tattccatct atgtgtacaa ggttctgaag caggtccacc ctgacaccgg catttcgtcc
      301 aaggccatgg gcatcatgaa ttcgtttgtg aacgacattt tcgagcgcat cgcaggtgag
      361 gcttcccgcc tggcgcatta caacaagcgc tcgaccatca cctccaggga gatccagacg
      421 gccgtgcgcc tgctgctgcc tggggagttg gccaagcacg ccgtgtccga gggtactaag
      481 gccatcacca agtacaccag cgctaagtaa ggatccaccg gatctagata actgatcata
      541 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      601 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      661 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      721 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
      781 taatattt
//
