LOCUS       sequence-160198-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfCherry2(11) tagged H2B in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pEGFP_sfCherry2(11)_H2B
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(137..514)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     promoter        complement(638..841)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(667..687)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc
      121 tagatccggt ggatccttac ttagcgctgg tgtacttggt gatggcctta gtaccctcgg
      181 acacggcgtg cttggccaac tccccaggca gcagcaggcg cacggccgtc tggatctccc
      241 tggaggtgat ggtcgagcgc ttgttgtaat gcgccaggcg ggaagcctca cctgcgatgc
      301 gctcgaaaat gtcgttcaca aacgaattca tgatgcccat ggccttggac gaaatgccgg
      361 tgtcagggtg gacctgcttc agaaccttgt acacatagat ggaatagctc tccttgcggc
      421 tgcgcttgcg cttcttgccg cctttcttct gcgccttagt caccgccttc ttggagccct
      481 ttttcggggc gggagcagac ttcgctggct ctgggcctcc ggagcctcca gatctgagcc
      541 ggacggtgct gtgtctggcc tcggctctct cgtactgctc cacgatggtg tacattggcg
      601 accggtagcg ctagcggatc tgacggttca ctaaaccagc tctgcttata tagacctccc
      661 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
      721 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
      781 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
      841 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtagga
//
