LOCUS       sequence-160203-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector for split sfCherry screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pET_sfCherry(1-10)_32aalinker_sfCherry(11)
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..91
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(231..249)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 ccacagcctg gaagttctgt tccaggggcc cggatccatg gaggaggaca acatggccat
      121 catcaaggag ttcatgagat tcaaggtgca catggagggc agcgtgaacg gccacgagtt
      181 cgagatcgag ggcgagggcg agggccaccc ctacgagggc acccagaccg ccaagctgaa
      241 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgagccccc agttcatgta
      301 cggcagcaag gcctacgtga agcaccccgc cgacatcccc gactacctga agctgagctt
      361 ccccgagggc ttcacctggg agagagtgat gaacttcgag gacggcggcg tggtgaccgt
      421 gacccaggac agcagcctgc aggacggcga gttcatctac aaggtgaagc tgctgggcac
      481 caacttcccc agcgacggcc ccgtgatgca gaagaagacc atgggctggg aggccagcac
      541 cgagagaatg taccccgagg acggcgccct gaagggcgag atcaaccaga gactgaagct
      601 gaaggacggc ggccactacg acgccgaggt gaagaccacc tacaaggcca agaagcccgt
      661 gcagctgccc ggcgcctaca acgtggacat caagctggac atcaccagcc acaacgagga
      721 cgacgttggt ggtggcggat cagaaggagg cggtagcgg
//
