LOCUS       sequence-160208-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces #61376. QUAS driving GFP with beta crystalline CFP eye 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  TITLE     QUAS:GFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Quas:GFPpA;Betacrystallin:CFP
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..79
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    730..754
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggaatgcgcg ttccaatgca ccgttcccgg caacctagca tgcagataac ttcgtataaa
       61 gtatcctata cgaagttatg gcaacgcgtt taaactacag ctgtgctggg aaggctggct
      121 cgctctggcc ttcctcacca ctgccgcagc ttttatacgc tggcaagggc agcacagcgg
      181 cgcgcggcgg acaatagaaa cacgccgtca tcagagttca gccgtgccga agcgcagggt
      241 catcgcatcg cccagaaggc cgtaagcatt ttactgtcaa tggaaagccc tgacggcggc
      301 actttccctc tcagcccgag ctctatgcta taggctgtat acctatggct aagagaggtc
      361 tgtgttcagg tcagaggtca cgtgatgata atccgagcag aggagtcgct cgctgataca
      421 ctccagagca gagtagattt catactgaag ttaataatgt agcatgacat atattagatg
      481 gtgtgtgtgt gtgtgtgtat atacatatat atatatatat ggtagcgtat ttagatggaa
      541 tttattgatg tattatttat tatatgtata tacagatgaa gtcaaaattt ttcgctccct
      601 gtatattttc cccttaaatc ctgtttaaga gaaagaaaca tatttctaat cataatagtt
      661 ttaataactc atttctaata actgatttct tctctctttg tcatgatgat agcaccaatt
      721 gctcgacggt ccactttgta caagaaagct gggtaaaaaa cctcccacac ctccccctga
      781 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      841 gttacaaata aagcaatagc atc
//
