LOCUS       sequence-160210-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses full length sfCherry2 and TagBFP fusion in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pSFFV_sfCherry2_TagBFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(83..784)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat caagcttgca tgcctgcagg
       61 tcgactctag agtcgcggcc gcttaattaa gcttgtgccc cagtttgcta gggaggtcgc
      121 agtatctggc cactgccacc tcgtgctgct cgacgtaggt ctcgttgttg gcctccttga
      181 ttctttccag tctgtagtcc acatagtaga cgccaggcat cttgaggttc ttagcgggtt
      241 tcttggatct atatgtggtc ttgatgtttg cgatcagatg gctcccgccc acgagcttca
      301 gggccatgtc gtttctgcct tccaggccgc cgtcagcggg gtacagcgtc tcggtgaagg
      361 cctcccagcc gagtgttttc ttctgcatca cagggccgtt ggatgtgaag ttcacccctc
      421 tgatcttgac gttgtagatg aggcagccgt cctggaggct ggtgtcctgg gtagcggtca
      481 gcacgccccc gtcttcgtat gtggtgactc tctcccatgt gaagccctca gggaaggact
      541 gcttgaagaa gtcggggatg ccctgggtgt ggttgatgaa ggtcttgctg ccgtagagga
      601 agctagtagc caggatgtcg aaggcgaagg ggagagggcc gccctcgacc accttgattc
      661 tcatggtctg ggtgccctcg tagggcttgc cttcgccctc ggatgtgcac ttgaagtgat
      721 ggttgtccac ggtgccctcc atgtacagct tcatgtgcat gttctcctta atcagctcgc
      781 tcatacttcc tggtgcagga ttcgaagcgc cgccggtgct gtgtctggcc tcggctctct
      841 cgtactgctc
//
