LOCUS       sequence-160215-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pEGFP_TOMM20_sfCherry2(11)
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      644..653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(682..733)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(711..731)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      121 gcggccgctt aggtgctgtg tctggcctcg gctctctcgt actgctccac gatggtgtac
      181 atggtggcga ccggtggatc cccgctaccg ccttccacat catcttcagc caagctctga
      241 gcacttacaa ttctctgact aattgttggg agcttagtca gaagcatctg gaacactggt
      301 ggtggaagag tttgctgtaa gacctgcagt aactgctgtg gctgtccaca cacagcaatt
      361 gcatttgtca gatggtctac gcccttctca tattcacctt gagctagtaa ctcttcacca
      421 agctgtattt cttcaaggaa gaacttctga acagcttcag catctttaag gtcaggtaac
      481 ttggaaagcc cagctctctc cttggcaagc ttctgtttct ttcttcgttc tcgaagcctg
      541 ttcttgaagt tggggtcact tcgtcttttg cggtcgaagt agatgcagta cccaatgaaa
      601 agggccccgc atacaccggc ggcgatggcg ctgttccgac ccaccatggt ggcgctagcg
      661 gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta cacgcctacc
      721 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgatt
//
