LOCUS       sequence-160217-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pEGFP_sfCherry2(11)_zyxin
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      75..84
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttcg aattccatgg cggccccccg cccgtctccc gcgatccccg tttcggtctc
      121 ggctccggct ttttacgccc cgcagaagaa gttcggccct gtggtggccc caaagcccaa
      181 agtgaatccc ttccggccca gggacagcga gcctcccccg gcacccgggg cccagcgcgc
      241 acagatgggc cgggtgggcg atattccccc gccgcccccg gaagactttc ccctgcctcc
      301 acctcccctt gctggggatg gcgacgatgc agagggtgct ctgggaggtg ccttcccgcc
      361 gccccctccc ccgatcgagg aatcatttcc ccctgcgcct ctggaggagg agatcttccc
      421 ttccccgccg cctcctccgg aggaggaggg agggcctgag gcccccatac cgcccccacc
      481 acagcccagg gagaaggtga gcagtattga tttggagatc gactctctgt cctcactgct
      541 ggatgacatg accaagaatg atcctttcaa agcccgggtg tcatctggat atgtgccccc
      601 accagtggcc actccattca gttccaagtc cagtaccaag cctgcagccg ggggcacagc
      661 acccctgcct ccttggaagt ccccttccag ctcccagcct ctgccccagg ttccggctcc
      721 ggctcagagc cagacacagt tccatgttca gccccagccc cagcccaagc ctcaggtcca
      781 actccatgtc cagtcccaga cccagcctgt gtctttggct a
//
