LOCUS       sequence-160220-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human CENP-A N-terminally labeled with LSSmOrange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Yoo TY, Choi JM, Conway W, Yu CH, Pappu RV, Needleman DJ
  TITLE     Measuring NDC80 binding reveals the molecular basis of 
            tension-dependent kinetochore-microtubule attachments.
  JOURNAL   Elife. 2018 Jul 25;7. pii: 36392. doi: 10.7554/eLife.36392.
  PUBMED    30044223
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-25"; volume: "7. pii: 36392. doi"; pages: " 
            10.7554/eLife.36392"
COMMENT     Sequence Label: pTY07
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..709
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     415..434
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 catggtgagc aagggcgagg agaataacat ggccatcatc aaggagttca tgcgcttcaa
       61 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      121 ccgcccctac gagggctttc agaccgttaa gctgaaggtg accaagggtg gccccctgcc
      181 cttcgcctgg gacatcttgt cccctcagtt cacctacggc tccaaggcct acgtgaagca
      241 ccccgccgac atccccgact acctcaagct gtccttcccc gagggcttca agtgggagcg
      301 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgact caggactcct ccctgcagga
      361 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
      421 aatgcagaag aagaccatgg gcatggaggc ctcctccgag cggatgtacc ccgaggacgg
      481 cgccctgaag ggcgaggaca agctcaggct gaagctgaag gacggcggcc actacacctc
      541 cgaggtcaag accacctaca aggccaagaa gcccgtgcag ttgcccggcg cctacatcgt
      601 cgacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
      661 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gaggaacatc
      721 cttcgcatct gcaacgaagt tatcagtcga caccatgggc ccgcgccgcc ggagccgaaa
      781 gcccgaggcc ccgaggaggc gcagcccgag cccgaccccg acc
//
