LOCUS       sequence-160227-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             131..166
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg ccaccatggc tactcaagct gacctgatgg agttggacat
      121 ggccatggag ccggacagaa aagctgctgt cagccactgg cagcagcagt cttacttgga
      181 ttctggaatc cattctggtg ccaccaccac agctccttcc ctgagtggca agggcaaccc
      241 tgaggaagaa gatgttgaca cctcccaagt cctttatgaa tgggagcaag gcttttccca
      301 gtccttcacg caagagcaag tagctgatat tgacgggcag tatgcaatga ctagggctca
      361 gagggtccga gctgccatgt tccctgagac gctagatgag ggcatgcaga tcccatccac
      421 gcagtttgac gctgctcatc ccactaatgt ccagcgcttg gctgaaccat cacagatgtt
      481 gaaacatgca gttgtcaatt tgattaacta tcaggatgac gcggaacttg ccacacgtgc
      541 aattcctgag ctgacaaaac tgctaaacga tgaggaccag gtggtagtta ataaagctgc
      601 tgttatggtc catcagcttt ccaaaaagga agcttccaga catgccatca tgcgctcccc
      661 tcagatggtg tctgccattg tacgcaccat gcagaataca aatgatgtag agacagctcg
      721 ttgtactgct gggactctgc acaacctttc tcaccaccgc gagggcttgc tggccatctt
      781 taagtctggt ggcatcccag cgctggtgaa
//
