LOCUS       sequence-160232-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For targeting shRNA construct into human AAVS1 locus, using genome 
            editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under
            U6 promoter, flanked by AAVS1 homology arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Daneshvar K, Pondick JV, Kim BM, Zhou C, York SR, Macklin JA, 
            Abualteen A, Tan B, Sigova AA, Marcho C, Tremblay KD, Mager J, Choi 
            MY, Mullen AC
  TITLE     DIGIT Is a Conserved Long Noncoding RNA that Regulates GSC 
            Expression to Control Definitive Endoderm Differentiation of 
            Embryonic Stem Cells.
  JOURNAL   Cell Rep. 2016 Oct 4;17(2):353-365. doi: 
            10.1016/j.celrep.2016.09.017.
  PUBMED    27705785
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-4- 4"; volume: "17"; issue: "2"; pages: "353-365"
COMMENT     Sequence Label: pAAVS1-shRNA
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     130..149
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
       61 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
      121 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
      181 tatatcttgt ggaaaggacg aaacaccggt gtgttgtaaa tgagcacaca aaatacacat
      241 gctaaaatat tatattctat gacctttata aaatcaacca aaatcttctt tttaataact
      301 ttagtatcaa taattagaat ttttatgttc ctttttgcaa acttttaata aaaatgagca
      361 aaataaaaaa acgctagttt tagtaactcg cgttgttttc ttcaccttta ataatagcta
      421 ctccaccact tgttcctaag cggtcagctc ctgcttcaat cattttttga gcatcttcaa
      481 atgttctaac tccaccagct gctttaacta aagcattgtc tttaacaact gacttcatta
      541 gtttaacatc ttcaaatgtt gcacctgatt ttgaaaatcc tgttgatgtt ttaacaaatt
      601 ctaatccagc ttcaacagct atttcacaag ctttcatgat ttcttctttt gttaataaac
      661 aattttccat aatacattta acaacatgtg atccagctgc tttttttaca gctttcatgt
      721 cttctaaaac taattcataa tttttgtctt ttaatgcacc aatatttaat accatatcaa
      781 tttctgttgc accatcttta attgcttcag aaacttcgaa tgcttttgta gctgttgtgc
      841 atgcacctag aggaaaacct acaacatttg ttattcctac atttgtgcct tttaataatt
      901 ctttacaata gcttgttcaa tatgaattaa cacaaactgt tgcaaaatca a
//
