LOCUS       sequence-160233-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For targeting shRNA construct into human AAVS1 locus, using genome 
            editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under
            U6 promoter, flanked by AAVS1 homology arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Daneshvar K, Pondick JV, Kim BM, Zhou C, York SR, Macklin JA, 
            Abualteen A, Tan B, Sigova AA, Marcho C, Tremblay KD, Mager J, Choi 
            MY, Mullen AC
  TITLE     DIGIT Is a Conserved Long Noncoding RNA that Regulates GSC 
            Expression to Control Definitive Endoderm Differentiation of 
            Embryonic Stem Cells.
  JOURNAL   Cell Rep. 2016 Oct 4;17(2):353-365. doi: 
            10.1016/j.celrep.2016.09.017.
  PUBMED    27705785
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-4- 4"; volume: "17"; issue: "2"; pages: "353-365"
COMMENT     Sequence Label: pAAVS1-shRNA
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    141..258
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcgccgctg cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg
       61 gctgccttgg aaaaggcgca accccaaaag ctttcgagcc gcggccaaag tggatctctg
      121 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
      181 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
      241 cccccttttc ttttaaaatt gtggatgaat actgccattt gtctcgaggt cgagaattct
      301 acaaaaaaga gcaagaagct aaaaaagatt taaaaattat ttttagcgca gttaatggaa
      361 caggaactaa atttacccca aaaatattac gtgaatcagg atataacgtt attgaggttg
      421 aagagcatgc atttgaagat gaaacattta aaaatgttgt aaatccaaat ccagaatttg
      481 atcctgcatg aaaaataccg cttgaatatg gtattaaaca tgatgcagat attattatta
      541 tgaatgaccc agatgctgac agatttggaa tggcaataaa acatgatggt cattttgtaa
      601 gattagatgg aaatcaaaca ggaccaattt taattgattg aaaattatca aatctaaaac
      661 gcttaaatag cattccaaaa aatccggctc tatattcaag ttttgtaaca agtgatttgg
      721 gtgatagaat cgctcatgaa aaatatggag ttaatattgt aaaaacttta actggattta
      781 aatgaatggg tagagaaatt gctaaagaag aagataacgg attaaatttt gtttttgctt
      841 atgaagaaag ttatggatat gtaattgatg actcagctag agataaagat ggaatacaag
      901 cttctatatt aatagcagag gctgcttgat tttataaaaa acaaaataaa acattagtag
      961 actattt
//
