LOCUS       sequence-160234-       10127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of WT L. shahii C2c2 CRISPR effector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian
            R, Doudna JA
  TITLE     Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA 
            processing and RNA detection.
  JOURNAL   Nature. 2016 Sep 26. doi: 10.1038/nature19802.
  PUBMED    27669025
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 26. doi: 10.1038/nature19802."
COMMENT     Sequence Label: p2CT-His-MBP-Lsh_C2c2_WT
FEATURES             Location/Qualifiers
     source          1..10127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..36)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        104..208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(209..277,278..1069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(427..446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1729..1748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1982..1999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2014..2156
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2082..2101)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2242..2264
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2258..2449)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3907..3926
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4010..4029
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4010..4028
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4117..4139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4159..4176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4186..5286
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5260..5283
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5341..5361
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             5368..9537
                     /codon_start=1
                     /product="type VI CRISPR effector from Leptotrichia shahii 
                     with RNA-guided RNAse activity (Abudayyeh et al., 2016)"
                     /label=LshC2c2
                     /translation="MGNLFGHKRWYEVRDKKDFKIKRKVKVKRNYDGNKYILNINENNN
                     KEKIDNNKFIRKYINYKKNDNILKEFTRKFHAGNILFKLKGKEGIIRIENNDDFLETEE
                     VVLYIEAYGKSEKLKALGITKKKIIDEAIRQGITKDDKKIEIKRQENEEEIEIDIRDEY
                     TNKTLNDCSIILRIIENDELETKKSIYEIFKNINMSLYKIIEKIIENETEKVFENRYYE
                     EHLREKLLKDDKIDVILTNFMEIREKIKSNLEILGFVKFYLNVGGDKKKSKNKKMLVEK
                     ILNINVDLTVEDIADFVIKELEFWNITKRIEKVKKVNNEFLEKRRNRTYIKSYVLLDKH
                     EKFKIERENKKDKIVKFFVENIKNNSIKEKIEKILAEFKIDELIKKLEKELKKGNCDTE
                     IFGIFKKHYKVNFDSKKFSKKSDEEKELYKIIYRYLKGRIEKILVNEQKVRLKKMEKIE
                     IEKILNESILSEKILKRVKQYTLEHIMYLGKLRHNDIDMTTVNTDDFSRLHAKEELDLE
                     LITFFASTNMELNKIFSRENINNDENIDFFGGDREKNYVLDKKILNSKIKIIRDLDFID
                     NKNNITNNFIRKFTKIGTNERNRILHAISKERDLQGTQDDYNKVINIIQNLKISDEEVS
                     KALNLDVVFKDKKNIITKINDIKISEENNNDIKYLPSFSKVLPEILNLYRNNPKNEPFD
                     TIETEKIVLNALIYVNKELYKKLILEDDLEENESKNIFLQELKKTLGNIDEIDENIIEN
                     YYKNAQISASKGNNKAIKKYQKKVIECYIGYLRKNYEELFDFSDFKMNIQEIKKQIKDI
                     NDNKTYERITVKTSDKTIVINDDFEYIISIFALLNSNAVINKIRNRFFATSVWLNTSEY
                     QNIIDILDEIMQLNTLRNECITENWNLNLEEFIQKMKEIEKDFDDFKIQTKKEIFNNYY
                     EDIKNNILTEFKDDINGCDVLEKKLEKIVIFDDETKFEIDKKSNILQDEQRKLSNINKK
                     DLKKKVDQYIKDKDQEIKSKILCRIIFNSDFLKKYKKEIDNLIEDMESENENKFQEIYY
                     PKERKNELYIYKKNLFLNIGNPNFDKIYGLISNDIKMADAKFLFNIDGKNIRKNKISEI
                     DAILKNLNDKLNGYSKEYKEKYIKKLKENDDFFAKNIQNKNYKSFEKDYNRVSEYKKIR
                     DLVEFNYLNKIESYLIDINWKLAIQMARFERDMHYIVNGLRELGIIKLSGYNTGISRAY
                     PKRNGSDGFYTTTAYYKFFDEESYKKFEKICYGFGIDLSENSEINKPENESIRNYISHF
                     YIVRNPFADYSIAEQIDRVSNLLSYSTRYNNSTYASVFEVFKKDVNLDYDELKKKFKLI
                     GNNDILERLMKPKKVSVLELESYNSDYIKNLIIELLTKIENTNDTL"
     primer_bind     complement(9663..9681)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9677..9724
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(10090..10095,10096..10112,10113..10118))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
     4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
     4141 atacatatga aatcttctca ccatcaccat caccatggtt cttctatgaa aatcgaagaa
     4201 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
     4261 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
     4321 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
     4381 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
     4441 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
     4501 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
     4561 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
     4621 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
     4681 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
     4741 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
     4801 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
     4861 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
     4921 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     4981 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     5041 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     5101 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc
     5161 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     5221 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     5281 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     5341 gaaaacctgt acttccaatc caatgcaatg gggaaccttt tcgggcataa acgctggtac
     5401 gaggtgcgcg ataagaagga ctttaagatc aaacgcaaag tcaaggttaa gcgtaattac
     5461 gacggtaaca aatacatctt aaacatcaac gagaacaaca ataaggagaa aatcgacaat
     5521 aacaagttta ttcgtaagta catcaattac aagaaaaacg acaatatcct taaagaattt
     5581 acgcgcaaat tccatgcagg caatatcttg ttcaagttaa agggtaagga ggggatcatt
     5641 cgtatcgaga acaatgatga ctttctggaa actgaagagg ttgtccttta catcgaagcg
     5701 tatgggaaga gcgagaagct gaaagctctg ggaatcacta aaaaaaaaat cattgacgag
     5761 gcgattcgtc aaggcatcac aaaggatgac aagaaaatcg aaatcaagcg ccaagagaac
     5821 gaagaagaaa tcgaaatcga catccgcgat gaatacacca acaagacctt aaatgattgc
     5881 agtattattc tgcgcatcat tgaaaacgat gagcttgaga cgaaaaagtc tatctatgag
     5941 atctttaaga atatcaacat gtcgttgtac aagattatcg agaagattat tgaaaatgag
     6001 actgagaaag tctttgaaaa ccgttattat gaagagcacc ttcgcgaaaa actgcttaag
     6061 gacgacaaaa ttgatgttat tctgactaac tttatggaga ttcgtgagaa gatcaagagc
     6121 aacctggaaa ttttagggtt cgtcaaattc tacttaaacg taggggggga taaaaagaag
     6181 tccaaaaaca agaagatgtt ggtggagaaa atcttaaaca tcaatgtaga cctgaccgtg
     6241 gaggacattg ctgattttgt tattaaggag ttggagtttt ggaacatcac aaaacgcatt
     6301 gaaaaagtta agaaagttaa taacgagttt ttagaaaagc gtcgcaaccg cacgtatatc
     6361 aaatcatacg tccttttaga taagcacgaa aaatttaaga tcgagcgcga aaacaagaaa
     6421 gataaaattg tcaaattttt cgtcgagaat atcaagaaca atagcatcaa ggagaagatc
     6481 gaaaagatct tggcggaatt taaaatcgac gaattgatca aaaaattaga gaaagagtta
     6541 aagaaaggta actgcgatac agagatcttc ggtatcttca agaaacacta taaggtcaat
     6601 tttgactcca agaagttctc caagaaatct gacgaggaaa aggaattgta taagattatc
     6661 tatcgctacc ttaaaggacg catcgagaag attctggtca acgaacagaa ggtacgctta
     6721 aagaagatgg agaaaatcga aattgagaag attttaaacg aatctatcct ttcggaaaaa
     6781 atccttaagc gcgtcaagca gtacaccctg gaacacatca tgtacttggg aaaacttcgt
     6841 cataatgata ttgatatgac gactgtcaat actgatgatt tttctcgctt acatgcgaaa
     6901 gaagagctgg acttggagtt gattacgttt ttcgcatcta cgaacatgga actgaacaag
     6961 attttctctc gcgagaatat taataacgac gaaaatattg acttctttgg aggtgaccgc
     7021 gaaaagaact atgtgctcga caagaaaatt ctcaactcta agattaagat cattcgcgat
     7081 cttgatttca tcgacaataa gaacaacatt acgaataact ttattcgcaa gtttaccaag
     7141 attggtacga atgagcgcaa tcgtattttg cacgccatta gtaaagaacg tgatttgcag
     7201 ggaacgcaag acgactataa caaggtaatc aacatcattc aaaacttgaa gatctccgac
     7261 gaagaagtat caaaagcctt gaatttggat gtcgtgttca aggataagaa gaacattatc
     7321 acaaaaatca acgatattaa aatcagtgag gagaataaca acgatattaa atatttaccg
     7381 agttttagca aggttctgcc ggaaatctta aatttatacc gcaataatcc taaaaacgaa
     7441 cccttcgata caattgagac tgaaaagatc gtgttgaacg ctcttattta tgtgaacaaa
     7501 gaattgtaca agaagttaat cctggaggat gacctggaag agaacgagag caaaaacatt
     7561 ttcttgcagg agctgaagaa aaccttaggt aatattgatg agatcgacga gaacatcatt
     7621 gagaattact acaagaacgc ccaaatctcg gcgtctaaag gaaataataa ggcgattaag
     7681 aaataccaga agaaagttat cgaatgctac atcgggtatt tgcgtaagaa ctacgaagag
     7741 cttttcgact tttcggactt taagatgaac atccaagaga ttaagaaaca aatcaaggat
     7801 attaatgaca ataagacgta tgagcgtatt acagtgaaaa ccagtgacaa gactatcgta
     7861 attaacgacg attttgagta catcatttca atctttgcgt tgttgaactc taatgccgta
     7921 atcaacaaga ttcgcaaccg tttcttcgcg acgtcagtgt ggctgaacac ctcagagtac
     7981 cagaatatta tcgatatcct ggacgagatt atgcagctga atacgctgcg taacgagtgt
     8041 attaccgaaa actggaacct caacttagaa gagtttatcc aaaagatgaa ggaaatcgag
     8101 aaggactttg atgattttaa aattcagaca aagaaggaga tctttaacaa ctattacgag
     8161 gacatcaaaa acaacatctt aacggaattt aaagacgata ttaacggttg cgacgtgctt
     8221 gaaaagaagt tggagaaaat cgtaatcttt gacgacgaaa ccaagtttga gatcgacaag
     8281 aagtccaata ttttgcagga cgagcagcgt aaattgagca atatcaataa gaaggacctg
     8341 aaaaagaagg tggaccaata tattaaagac aaggatcagg aaatcaaatc gaagattttg
     8401 tgccgtatta tcttcaactc agacttcctt aagaaatata agaaggaaat tgacaatttg
     8461 atcgaagata tggagtctga gaatgagaac aagttccaag agatttatta cccgaaagag
     8521 cgcaagaacg aactctatat ctataagaaa aacttgttct taaatattgg aaacccaaac
     8581 tttgacaaga tttatggcct tatttcgaac gatattaaga tggccgatgc taaattcttg
     8641 tttaatatcg atggtaagaa tattcgtaaa aataagatta gtgaaattga cgccattttg
     8701 aagaacctta acgacaagct gaacggctac tcgaaagagt acaaggagaa gtatatcaaa
     8761 aagttgaagg aaaacgatga cttctttgcc aaaaatatcc agaacaaaaa ttacaagagc
     8821 ttcgaaaagg attacaaccg cgtttctgag tataaaaaga tccgcgacct tgtagagttc
     8881 aactatttaa acaaaatcga atcttactta atcgatatca actggaaatt ggcgattcaa
     8941 atggcccgct ttgagcgcga catgcactat atcgtaaatg ggttgcgcga gttgggaatt
     9001 atcaagttgt caggatataa cacggggatc tcacgtgcat acccaaagcg caatggatca
     9061 gacgggtttt acacaaccac tgcttactat aagttctttg atgaggaatc ctacaagaaa
     9121 ttcgaaaaga tctgttacgg tttcggtatc gacttgagcg aaaacagcga gatcaacaag
     9181 ccagaaaatg aatccattcg caactatatt tctcactttt atatcgttcg caacccgttt
     9241 gcagactact ctattgcgga gcagatcgac cgtgtgtcga atttgctgag ttatagcacc
     9301 cgctataaca actcgacgta tgcatcagta ttcgaggtct ttaaaaagga cgtgaacctt
     9361 gactatgatg aattaaagaa aaaattcaag cttattggca ataatgacat cttggaacgc
     9421 ttgatgaagc cgaagaaagt ctccgtactt gaattagaat cgtacaactc tgattacatc
     9481 aagaacctga tcattgagtt attgactaag attgaaaata caaacgatac cctgtaataa
     9541 cattggaagt ggataacgga tccgcgatcg cggcgcgcca cctggtggcc ggccggtacc
     9601 acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     9661 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     9721 tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg
     9781 cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt
     9841 cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca
     9901 gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca
     9961 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
    10021 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
    10081 aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
//
