LOCUS       sequence-160240-         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression vector for Venus-MDGA1 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: pCAG-Venus-MDGA1
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      396..405
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(434..453)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(515..537)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cgggcgaagc actgcaggcc gtagcccagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc
      181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgctagcggc
      301 gggcagcatc tgaaagcagg cccccagcag acacaagacc acccccgagg gcctcctcag
      361 cagccagtat agacagagac cgagcagcag gaaatccatg gtggcggaat tctttgccaa
      421 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
      481 catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg
      541 ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga ggctggagag
      601 ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt tccttcctgc
      661 cggc
//
