LOCUS       sequence-160245-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression vector for Venus-MDGA2 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: pCAG-Venus-MDGA2
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..237
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      387..396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(425..444)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(506..528)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
       61 gcgggcgaag cactgcaggc cgtagcccag ggtggtcacg agggtgggcc agggcacggg
      121 cagcttgccg gtggtgcaga tcagcttcag ggtcagcttg ccgtaggtgg catcgccctc
      181 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      241 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atgctagcgg cgggcagcat
      301 ctgaaagcag gcccccagca gacacaagac cacccccgag ggcctcctca gcagccagta
      361 tagacagaga ccgagcagca ggaaatccat ggtggcggaa ttctttgcca aaatgatgag
      421 acagcacaat aaccagcacg ttgcccagga gctgtaggaa aaagaagaag gcatgaacat
      481 ggttagcaga ggctctagag ccgccggtca cacgccagaa gccgaacccc gccctgcccc
      541 gtcccccccg aaggcagccg tccccccgcg gacagccccg aggctggaga gggagaaggg
      601 gacggcggcg cggcgacgca cgaaggccct ccccgcccat ttccttcctg ccggcgccgc
      661 accgcttcgc cc
//
