LOCUS       sequence-160256-        3090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway donor vector for IAA15 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA15_no stop
FEATURES             Location/Qualifiers
     source          1..3090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1215..1314)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1331..1350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1332..1350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1355..1371)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1355..1371)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1473..1492)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1484..2293
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1551..1570)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2440..3028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2929..2948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc aaaatgtcac cggaggaata cgttagggtt tggccggatt ccggtgatct
      721 tggaggaact gagctgaccc tagctttgcc tggcactccg acaaatgcat cggaaggtcc
      781 aaaaaagttc gggaacaaac gtagattcct cgagaccgtt gatttaaaac tcggggaagc
      841 acacgagaac aattatatct caagcatggt cactaatgac cagttggtgg gctggccgcc
      901 ggtagcgaca gcgaggaaaa cagtgaggcg gaaatacgtg aaagtggcgt tggatggtgc
      961 ggcctatctc agaaaggttg atcttgggat gtacgattgc tatggacagc ttttcaccgc
     1021 tctagaaaac atgtttcagg ggataataac aatatgtaga gtgacagagt tggagaggaa
     1081 gggagagttt gttgctactt acgaggataa ggacggtgac ttgatgttag tcggagatgt
     1141 gccgtggatg atgtttgtgg aatcttgcaa acgtatgagg ttaatgaaaa ccggagatgc
     1201 tattggatta tagcacccag ctttcttgta caaagttggc attataagaa agcattgctt
     1261 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     1321 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     1381 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac
     1441 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     1501 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     1561 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     1621 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     1681 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     1741 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     1801 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     1861 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     1921 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     1981 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     2041 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     2101 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     2161 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     2221 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     2281 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     2341 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     2401 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2461 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2521 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2581 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2641 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2701 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2761 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2821 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2881 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2941 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3001 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3061 ggccttttgc tggccttttg ctcacatgtt
//
