LOCUS       sequence-160282-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  binary DEST vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  TITLE     pAM-PAT-ProUBQ10 GW
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAM-PAT-35S-GW-Luc
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..549)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(591..770)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 gatttcggtg acgggcagga ccggacgggg cggcaccggc aggctgaagt ccagctgcca
       61 gaaacccacg tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg
      121 ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc
      181 gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga
      241 gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc
      301 gtaggcgttg cgtgccttcc agggacccgc gtaggcgatg ccggcgacct cgccgtccac
      361 ctcggcgacg agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg
      421 cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat
      481 ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc
      541 tgggctcatg gtagatcccc tcgatcgagt tgagagtgaa tatgagactc taattggata
      601 ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga
      661 tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc
      721 attaaactcc agaaacccgc ggctcagtgg ctccttcaac gttgcggttc tgtcagttcc
      781 aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc
      841 tccgctcatg atcagattgt cgtttcccgc cttcggtttg ggcgcgcccg gttcagaaga
      901 ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
      961 ttgcccagct atctgtcact tcatcgaa
//
