LOCUS       sequence-160289-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cyan-green fluorescent protein with tryptophan-based chromophore in 
            equilibrium between anionic and neutral states..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Sarkisyan KS, Yampolsky IV, Solntsev KM, Lukyanov SA, Lukyanov KA, 
            Mishin AS
  TITLE     Tryptophan-based chromophore in fluorescent proteins can be anionic.
  JOURNAL   Sci Rep. 2012;2:608. doi: 10.1038/srep00608. Epub 2012 Aug 29.
  PUBMED    22934131
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2012"; volume: "2:608. doi"; pages: " 10.1038/srep00608. Epub
            2012 Aug 29"
COMMENT     Sequence Label: WasCFP /pQE-30
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(50..763,764..766))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLKTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISGNVNITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKQSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(95..116)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     444..463
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     703..724
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(773..790)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             809..820
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    839..855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(863..869,870..886,887..892,893..907))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    871..887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(931..949)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtcattactg gatctatcaa caggagtcca agctcagcta attaagcttt tacttgtaca
       61 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
      121 cgcgcttctc gttggggtct ttgctctgct tggactgggt gctcaggtag tggttgtcgg
      181 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
      241 cgctgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct
      301 tgtcggcggt gatattgacg ttgccgctga tggcgttgta ctccagcttg tgccccagga
      361 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
      421 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
      481 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
      541 cggggtagcg ggcgaagcac tgcacgcccc aggtcagggt ggttttgagg gtgggccagg
      601 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
      661 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
      721 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcacggat ccgtgatggt
      781 gatggtgatg cgatcctctc atagttaatt tctcctcttt aatgaattct gtgtgaaatt
      841 gttatccgct cacaattgaa tctattataa ttgttatccg ctcacaaagc aaataaattt
      901 tttatgattt ctcgaggtga agacgaaagg gcctcgtgat acgcctattt ttataggtta
      961 atgtcatgat aataatggtt tcttagacgt
//
