LOCUS       sequence-160293-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ150 - pDESTttTi5605[R4-R3]
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(97..103,104..121,122..127))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(152..275)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(283..300)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(283..299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
       61 ctgaaaatct cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa
      121 gtgtaaagcc tggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca
      181 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
      241 ttacgtttct cgttcaactt tattatacat agttgataat tcactggccg tcgttttaca
      301 ctcgagacgt acggtgcgcg cgatgcattc gaagatctgc ccactagtga gtcgtattat
      361 aagtgcaagt aagatcagtg tttgtttcga tatcagtctg tttcgtaacg gtcttctgta
      421 taactacaaa aaagaataaa aaaccgtatc acaactgaga aaaaagacaa aacttattga
      481 acagtgttcc tttcaaaaaa ttattttgta aaaaatcgag tttttctcca aaataacgca
      541 cgcaatcaaa aacaagaaaa ctaacgtgtt ctcctgggcg tggaactcct tatcaagatt
      601 accattattg tctgtcagaa ttgatatttc aattatttca tatctagaca aacccgggtg
      661 tcttgcattt attagtactg tgagtttaca tacaaacgag gcttactcag ataacataga
      721 gtcacctcta attagcaaaa acccatttct aattagctat ttcgaccagt tctaaagtca
      781 ccgtaattag aaatggtttt tgtagctttt ctaattagaa atgaccccga gataagtgaa
      841 atatggcatt ttctgggttt tctagtaaga aatgatcc
//
