LOCUS       sequence-160297-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Longitudinal monitoring of Gaussia and Nano luciferase activities to
            simultaneously assess ER calcium homeostasis and ER stress in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Wires ES, Henderson MJ, Yan X, Back S, Trychta KA, Lutrey MH, Harvey
            BK
  TITLE     Longitudinal monitoring of Gaussia and Nano luciferase activities to
            concurrently assess ER calcium homeostasis and ER stress in vivo.
  JOURNAL   PLoS One. 2017 Apr 12;12(4):e0175481. doi: 
            10.1371/journal.pone.0175481. eCollection 2017.
  PUBMED    28403212
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-12"; volume: "12(4):e0175481. doi"; pages: " 
            10.1371/journal.pone.0175481. eCollection 2017"
COMMENT     Sequence Label: pOTTC840 - pAAV 5xATF6 secNLuc
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..586)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcgg cgcgccactc
       61 tagagtcgcg gccttacgcc agaatgcgtt cgcacagccg ccagccggtc actccgttga
      121 tggttactcg gaacagcagg gagccgtcgg ggttgatcag gcgctcgtcg ataattttgt
      181 tgccgttcca cagggtccct gttacagtga tctttttgcc gtcgaacacg gcgatgcctt
      241 catacggccg tccgaaatag tcgatcatgt tcggcgtaac cccgtcgatt accagtgtgc
      301 catagtgcag gatcacctta aagtgatgat catccacagg gtacaccacc ttaaaaattt
      361 tttcgatctg gcccatttgg tcgccgctca gaccttcata cgggatgatg acatggatgt
      421 cgatcttcag cccattttca ccgctcagga caatcctttg gatcggagtt acggacaccc
      481 cgagattctg aaacaaactg gacacacctc cctgttcaag gacttggtcc aggttgtagc
      541 cggctgtctg tcgccagtcc ccaacgaaat cttcgagtgt gaagactggg gcagggaagg
      601 cagcaggcaa caccaggagc aggcccaggg agaaggcaac tggaccgaag gcgcttgtgg
      661 agaaggagtt catggtggct ttaccaacag taccggccgc cggctcagtc ttggcttctc
      721 agttgctcgc tgcagatgcg gttggagtac gaggcgccgc agccactgct tttataacaa
      781 gcgttttatg aatgagtgca gatctcgagg aatcgccacg tcagcacctg tctcgaggaa
      841 tcgccacgtc agcacctgtc tcgaggaatc gccacgtcag cacctgtctc gaggaatcgc
      901 cacgtcagca cctgtctcga ggaatcgcca cgtcagcacc tgtctcgagc aagcttgggc
      961 tgcaacgcgt gcggccgcag gaa
//
