LOCUS       sequence-160301-       11310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the OSKM 
            polycistronic reprogramming cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.02.004"; journalName: "Stem Cell Reports"; 
            date: "2015-04-14- 14"; volume: "4"; issue: "4"; pages: "727-43"
COMMENT     Sequence Label: PB-TAC-OSKM
FEATURES             Location/Qualifiers
     source          1..11310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   711..773
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1444..1468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2690..2746
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3725..3778
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5249..5308
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     protein_bind    complement(6720..6744)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6786..7360,7361..7363,7364..7372)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6953..6970)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7180..7199
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7373..8083
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7518..7536)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7786..7805
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8095..8114)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8160..8215
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8214..8233)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   8935..8969
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9119..9137)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9158..9174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9158..9174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9171..9193)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9182..9198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9206..9212,9213..9230,9231..9236))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9251..9272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9389..9406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9560..10148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9640..9659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10319..11110,11111..11179))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10942..10961
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11180..11284)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcgccat
     1621 ggctggacac ctggcttcag acttcgcctt ctcaccccca ccaggtgggg gtgatgggtc
     1681 agcagggctg gagccgggct gggtggatcc tcgaacctgg ctaagcttcc aagggcctcc
     1741 aggtgggcct ggaatcggac caggctcaga ggtattgggg atctccccat gtccgcccgc
     1801 atacgagttc tgcggaggga tggcatactg tggacctcag gttggactgg gcctagtccc
     1861 ccaagttggc gtggagactt tgcagcctga gggccaggca ggagcacgag tggaaagcaa
     1921 ctcagaggga acctcctctg agccctgtgc cgaccgcccc aatgccgtga agttggagaa
     1981 ggtggaacca actcccgagg agtcccagga catgaaagcc ctgcagaagg agctagaaca
     2041 gtttgccaag ctgctgaagc agaagaggat caccttgggg tacacccagg ccgacgtggg
     2101 gctcaccctg ggcgttctct ttggaaaggt gttcagccag accaccatct gtcgcttcga
     2161 ggccttgcag ctcagcctta agaacatgtg taagctgcgg cccctgctgg agaagtgggt
     2221 ggaggaagcc gacaacaatg agaaccttca ggagatatgc aaatcggaga ccctggtgca
     2281 ggcccggaag agaaagcgaa ctagcattga gaaccgtgtg aggtggagtc tggagaccat
     2341 gtttctgaag tgcccgaagc cctccctaca gcagatcact cacatcgcca atcagcttgg
     2401 gctagagaag gatgtggttc gagtatggtt ctgtaaccgg cgccagaagg gcaaaagatc
     2461 aagtattgag tattcccaac gagaagagta tgaggctaca gggacacctt tcccaggggg
     2521 ggctgtatcc tttcctctgc ccccaggtcc ccactttggc accccaggct atggaagccc
     2581 ccacttcacc acactctact cagtcccttt tcctgagggc gaggcctttc cctctgttcc
     2641 cgtcactgct ctgggctctc ccatgcattc aaacgctagc ggcagcggcg ccacgaactt
     2701 ctctctgtta aagcaagcag gagatgttga agaaaacccc gggcctgcat gcatgtataa
     2761 catgatggag acggagctga agccgccggg cccgcagcaa gcttcggggg gcggcggcgg
     2821 aggaggcaac gccacggcgg cggcgaccgg cggcaaccag aagaacagcc cggaccgcgt
     2881 caagaggccc atgaacgcct tcatggtatg gtcccggggg cagcggcgta agatggccca
     2941 ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgcgg agtggaaact
     3001 tttgtccgag accgagaagc ggccgttcat cgacgaggcc aagcggctgc gcgctctgca
     3061 catgaaggag cacccggatt ataaataccg gccgcggcgg aaaaccaaga cgctcatgaa
     3121 gaaggataag tacacgcttc ccggaggctt gctggccccc ggcgggaaca gcatggcgag
     3181 cggggttggg gtgggcgccg gcctgggtgc gggcgtgaac cagcgcatgg acagctacgc
     3241 gcacatgaac ggctggagca acggcagcta cagcatgatg caggagcagc tgggctaccc
     3301 gcagcacccg ggcctcaacg ctcacggcgc ggcacagatg caaccgatgc accgctacga
     3361 cgtcagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga acggctcgcc
     3421 cacctacagc atgtcctact cgcagcaggg cacccccggt atggcgctgg gctccatggg
     3481 ctctgtggtc aagtccgagg ccagctccag cccccccgtg gttacctctt cctcccactc
     3541 cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtacc tccccggcgc
     3601 cgaggtgccg gagcccgctg cgcccagtag actgcacatg gcccagcact accagagcgg
     3661 cccggtgccc ggcacggcca ttaacggcac actgcccctg tcgcacatgg catgcggctc
     3721 cggcgagggc aggggaagtc ttctaacatg cggggacgtg gaggaaaatc ccggcccact
     3781 cgagatgagg cagccacctg gcgagtctga catggctgtc agcgacgctc tgctcccgtc
     3841 cttctccacg ttcgcgtccg gcccggcggg aagggagaag acactgcgtc cagcaggtgc
     3901 cccgactaac cgttggcgtg aggaactctc tcacatgaag cgacttcccc cacttcccgg
     3961 ccgcccctac gacctggcgg cgacggtggc cacagacctg gagagtggcg gagctggtgc
     4021 agcttgcagc agtaacaacc cggccctcct agcccggagg gagaccgagg agttcaacga
     4081 cctcctggac ctagacttta tcctttccaa ctcgctaacc caccaggaat cggtggccgc
     4141 caccgtgacc acctcggcgt cagcttcatc ctcgtcttcc ccagcgagca gcggccctgc
     4201 cagcgcgccc tccacctgca gcttcagcta tccgatccgg gccgggggtg acccgggcgt
     4261 ggctgccagc aacacaggtg gagggctcct ctacagccga gaatctgcgc cacctcccac
     4321 ggcccccttc aacctggcgg acatcaatga cgtgagcccc tcgggcggct tcgtggctga
     4381 gctcctgcgg ccggagttgg acccagtata cattccgcca cagcagcctc agccgccagg
     4441 tggcgggctg atgggcaagt ttgtgctgaa ggcgtctctg accacccctg gcagcgagta
     4501 cagcagccct tcggtcatca gtgttagcaa aggaagccca gacggcagcc accccgtggt
     4561 agtggcgccc tacagcggtg gcccgccgcg catgtgcccc aagattaagc aagaggcggt
     4621 cccgtcctgc acggtcagcc ggtccctaga ggcccatttg agcgctggac cccagctcag
     4681 caacggccac cggcccaaca cacacgactt ccccctgggg cggcagctcc ccaccaggac
     4741 tacccctaca ctgagtcccg aggaactgct gaacagcagg gactgtcacc ctggcctgcc
     4801 tcttccccca ggattccatc cccatccggg gcccaactac cctcctttcc tgccagacca
     4861 gatgcagtca caagtcccct ctctccatta tcaagagctc atgccaccgg gttcctgcct
     4921 gccagaggag cccaagccaa agaggggaag aaggtcgtgg ccccggaaaa gaacagccac
     4981 ccacacttgt gactatgcag gctgtggcaa aacctatacc aagagttctc atctcaaggc
     5041 acacctgcga actcacacag gcgagaaacc ttaccactgt gactgggacg gctgtgggtg
     5101 gaaattcgcc cgctccgatg aactgaccag gcactaccgc aaacacacag ggcaccggcc
     5161 ctttcagtgc cagaagtgcg acagggcctt ttccaggtcg gaccaccttg ccttacacat
     5221 gaagaggcac tttctcgagg gctcgggcca gtgtactaat tatgctctct tgaaattggc
     5281 tggagatgtt gagagcaacc caggtcccgg cgcgccactg gatttccttt gggcgttgga
     5341 aaccccgcag acagccacga cgatgcccct caacgtgaac ttcaccaaca ggaactatga
     5401 cctcgactac gactccgtac agccctattt catctgcgac gaggaagaga atttctatca
     5461 ccagcaacag cagagcgagc tgcagccgcc cgcgcccagt gaggatatct ggaagaaatt
     5521 cgagctgctt cccaccccgc ccctgtcccc gagccgccgc tccgggctct gctctccatc
     5581 ctatgttgcg gtcgctacgt ccttctcccc aagggaagac gatgacggcg gcggtggcaa
     5641 cttctccacc gccgatcagc tggagatgat gaccgagtta cttggaggag acatggtgaa
     5701 ccagagcttc atctgcgatc ctgacgacga gaccttcatc aagaacatca tcatccagga
     5761 ctgtatgtgg agcggtttct cagccgctgc caagctggtc tcggagaagc tggcctccta
     5821 ccaggctgcg cgcaaagaca gcaccagcct gagccccgcc cgcgggcaca gcgtctgctc
     5881 cacctccagc ctgtacctgc aggacctcac cgccgccgcg tccgagtgca ttgacccctc
     5941 agtggtcttt ccctacccgc tcaacgacag cagctcgccc aaatcctgta cctcgtccga
     6001 ttccacggcc ttctctcctt cctcggactc gctgctgtcc tccgagtcct ccccacgggc
     6061 cagccctgag cccctagtgc tgcatgagga gacaccgccc accaccagca gcgactctga
     6121 agaagagcaa gaagatgagg aagaaattga tgtggtgtct gtggagaaga ggcaaacccc
     6181 tgccaagagg tcggagtcgg gctcatctcc atcccgaggc cacagcaaac ctccgcacag
     6241 cccactggtc ctcaagaggt gccacgtctc cactcaccag cacaactacg ccgcaccccc
     6301 ctccacaagg aaggactatc cagctgccaa gagggccaag ttggacagtg gcagggtcct
     6361 gaagcagatc agcaacaacc gcaagtgctc cagccccagg tcctcagaca cggaggaaaa
     6421 cgacaagagg cggacacaca acgtcttgga acgtcagagg aggaacgagc tgaagcgcag
     6481 cttttttgcc ctgcgtgacc agatccctga attggaaaac aacgaaaagg cccccaaggt
     6541 agtgatcctc aaaaaagcca ccgcctacat cctgtccatt caagcagacg agcacaagct
     6601 cacctctgaa aaggacttat tgaggaaacg acgagaacag ttgaaacaca aactcgaaca
     6661 gcttcgaaac tctggtgcat aaggcgcgcc gaattcgcgg ccgcactcga gatatctaga
     6721 cccagctttc ttgtacaaag tggtgatcta gctaaaacgc ggcctcgaat cgaattccgc
     6781 ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     6841 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     6901 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     6961 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     7021 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     7081 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     7141 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     7201 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     7261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     7321 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag
     7381 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     7441 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     7501 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     7561 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     7621 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     7681 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     7741 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     7801 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     7861 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     7921 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     7981 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     8041 gggccgccac tccaccggcg gcatggacga gctgtacaag taaagcggcc gcgatctttt
     8101 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     8161 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     8221 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     8281 ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt
     8341 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     8401 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     8461 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     8521 ctcttctctt atgaagatcc ctcgacctgc aggaattcga tatcaagctc tagccaacaa
     8581 gctcgtcatc gctttgcaga agagcagaga ggatatgctc atcgtctaaa gaactaccca
     8641 ttttattata tattagtcac gatatctata acaagaaaat atatatataa taagttatca
     8701 cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg
     8761 taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca
     8821 cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa
     8881 tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg
     8941 tcaattttac gcagactatc tttctagggt taaaaaagat ttgcgcttta ctcgacctaa
     9001 actttaaaca cgtcatagaa tcttcgtttg acaaaaacca cattgtggcc aagctgtgtg
     9061 acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga ggtacccagc ttttgttccc
     9121 tttagtgagg gttaattccg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9181 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9241 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9301 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9361 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9541 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    10021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10201 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10261 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10321 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10381 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10441 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10501 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10561 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10621 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10681 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10741 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10801 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10861 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10921 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10981 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11041 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11101 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11161 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11221 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11281 cgcgcacatt tccccgaaaa gtgccacctg
//
