LOCUS       sequence-160303-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing , part of the Target Accelerator 
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.D162E
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             134..169
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
       61 gccaactttg tacaaaaaag ttggcaccat ggctactcaa gctgatttga tggagttgga
      121 catggccatg gaaccagaca gaaaagcggc tgttagtcac tggcagcaac agtcttacct
      181 ggactctgga atccattctg gtgccactac cacagctcct tctctgagtg gtaaaggcaa
      241 tcctgaggaa gaggatgtgg atacctccca agtcctgtat gagtgggaac agggattttc
      301 tcagtccttc actcaagaac aagtagctga tattgatgga cagtatgcaa tgactcgagc
      361 tcagagggta cgagctgcta tgttccctga gacattagat gagggcatgc agatcccatc
      421 tacacagttt gatgctgctc atcccactaa tgtccagcgt ttggctgaac catcacagat
      481 gctgaaacat gcagttgtaa acttgattaa ctatcaagat gatgcagaac ttgccacacg
      541 tgcaatccct gaactgacaa aactgctaaa tgaagaggac caggtggtgg ttaataaggc
      601 tgcagttatg gtccatcagc tttctaaaaa ggaagcttcc agacacgcta tcatgcgttc
      661 tcctcagatg gtgtctgcta ttgtacgtac catgcagaat acaaatgatg tagaaacagc
      721 tcgttgtacc gctgggacct tgcataacct ttcccatcat cgtgagggct tactggccat
      781 ctttaagtct ggaggcattc ctgccctggt gaaaatgctt ggttcaccag tggattctgt
      841 gttgttttat gccattacaa ctctccacaa ccttttatta catcaagaag gagctaaaat
      901 ggcagtgcgt ttagctggtg ggctgcagaa aatggttgcc ttgctcaaca aaacaaatgt
      961 taaattcttg gctattacga cagactgcct tcaaatttta gcttat
//
