LOCUS       sequence-160304-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing EGFR , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_EGFR_p.A289V
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
       61 aatgccaact ttgtacaaaa aagttggcac catgcgaccc tccgggacgg ccggggcagc
      121 gctcctggcg ctgctggctg cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt
      181 ttgccaaggc acgagtaaca agctcacgca gttgggcact tttgaagatc attttctcag
      241 cctccagagg atgttcaata actgtgaggt ggtccttggg aatttggaaa ttacctatgt
      301 gcagaggaat tatgatcttt ccttcttaaa gaccatccag gaggtggctg gttatgtcct
      361 cattgccctc aacacagtgg agcgaattcc tttggaaaac ctgcagatca tcagaggaaa
      421 tatgtactac gaaaattcct atgccttagc agtcttatct aactatgatg caaataaaac
      481 cggactgaag gagctgccca tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt
      541 cagcaacaac cctgccctgt gcaacgtgga gagcatccag tggcgggaca tagtcagcag
      601 tgactttctc agcaacatgt cgatggactt ccagaaccac ctgggcagct gccaaaagtg
      661 tgatccaagc tgtcccaatg ggagctgctg gggtgcagga gaggagaact gccagaaact
      721 gaccaaaatc atctgtgccc agcagtgctc cgggcgctgc cgtggcaagt cccccagtga
      781 ctgctgccac aaccagtgtg ctgcaggctg cacaggcccc cgggagagcg actgcctggt
      841 ctgccgcaaa ttccgagacg aagccacgtg caaggacacc tgccccccac tcatgctcta
      901 caaccccacc acgtaccaga tggatgtgaa ccccgagggc aaatacagct ttggtgtcac
      961 ctgcgtgaag aagtgtcccc gtaattatgt ggtgacagat cacggctcgt gcgtccgagc
     1021 ctgtggggcc gacagc
//
