LOCUS       sequence-160308-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_WT
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             144..179
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 atatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag ttggcaccat ggctactcaa gctgatttga
      121 tggagttgga catggccatg gaaccagaca gaaaagcggc tgttagtcac tggcagcaac
      181 agtcttacct ggactctgga atccattctg gtgccactac cacagctcct tctctgagtg
      241 gtaaaggcaa tcctgaggaa gaggatgtgg atacctccca agtcctgtat gagtgggaac
      301 agggattttc tcagtccttc actcaagaac aagtagctga tattgatgga cagtatgcaa
      361 tgactcgagc tcagagggta cgagctgcta tgttccctga gacattagat gagggcatgc
      421 agatcccatc tacacagttt gatgctgctc atcccactaa tgtccagcgt ttggctgaac
      481 catcacagat gctgaaacat gcagttgtaa acttgattaa ctatcaagat gatgcagaac
      541 ttgccacacg tgcaatccct gaactgacaa aactgctaaa tgacgaggac caggtggtgg
      601 ttaataaggc tgcagttatg gtccatcagc tttctaaaaa ggaagcttcc agacacgcta
      661 tcatgcgttc tcctcagatg gtgtctgcta ttgtacgtac catgcagaat acaaatgatg
      721 tagaaacagc tcgttgtacc gctgggacct tgcataacct ttcccatcat cgtgagggct
      781 tactggccat ctttaagtct ggaggcattc ctgccctggt gaaaatgctt ggttcaccag
      841 tggattctgt gttgttttat gccattacaa ctctccacaa ccttttatta catcaagaag
      901 gagctaaaat ggcagtgcgt ttagctggtg ggctgcagaa aatggttgcc ttgctcaaca
      961 aaacaaatgt taaattcttg gctattacga cagactgcct tcaaatttta gcttatggca
     1021 accaagaaag caagctcatc atac
//
