LOCUS       sequence-160316-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PTEN , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PTEN_WT
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca ccatgacagc catcatcaaa gagatcgtta
      121 gcagaaacaa aaggagatat caagaggatg gattcgactt agacttgacc tatatttatc
      181 caaacattat tgctatggga tttcctgcag aaagacttga aggcgtatac aggaacaata
      241 ttgatgatgt agtaaggttt ttggattcaa agcataaaaa ccattacaag atatacaatc
      301 tttgtgctga aagacattat gacaccgcca aatttaattg cagagttgca caatatcctt
      361 ttgaagacca taacccacca cagctagaac ttatcaaacc cttttgtgaa gatcttgacc
      421 aatggctaag tgaagatgac aatcatgttg cagcaattca ctgtaaagct ggaaagggac
      481 gaactggtgt aatgatatgt gcatatttat tacatcgggg caaattttta aaggcacaag
      541 aggccctaga tttctatggg gaagtaagga ccagagacaa aaagggagta actattccca
      601 gtcagaggcg ctatgtgtat tattatagct acctgttaaa gaatcatctg gattatagac
      661 cagtggcact gttgtttcac aagatgatgt ttgaaactat tccaatgttc agtggcggaa
      721 cttgcaatcc tcagtttgtg gtctgccagc taaaggtgaa gatatattcc tccaattcag
      781 gacccacacg acgggaagac aagttcatgt actttgagtt ccctcagccg ttacctgtgt
      841 gtggtgatat caaagtagag ttcttcc
//
