LOCUS       sequence-160318-        7356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic 
            interneurons under the control of the hDlx enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu 
            Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith 
            GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, 
            Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, 
            Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G,
            Fishell G
  TITLE     A viral strategy for targeting and manipulating interneurons across 
            vertebrate species.
  JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.
  PUBMED    27798629
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430."
COMMENT     Sequence Label: pAAV-hDlx-GqDREADD-dTomato-Fishell-4
FEATURES             Location/Qualifiers
     source          1..7356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     intron          860..992
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     975..994
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1044..1070
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2859..2915
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2928..2948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2949..3647
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(3079..3097)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3347..3366
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3541..3564
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3702..4290
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3755..3775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4173..4184)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4459..4540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4496..4515)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4550..4569
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4619..4759
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4619..4748
                     /label=AAV2 ITR
     rep_origin      4834..5289
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4921..4940)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5131..5152
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5306..5325)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5425..5447
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5571..5675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5676..5744,5745..6536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5894..5913)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6707..7295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7196..7215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttatt cagaatgtta tgcactcaca
      181 gtggtttggc atgcatctgg tgaatttttt ttaacgaaaa attagtgttg gtttcgatgt
      241 atggtagcat tctccctaac gtaatttgaa taattcagca aagccccact accagctgta
      301 cttctgcagc ctcttccatt cttttcagca ttataatttt ggttaatttt caattttagg
      361 tcctacgtct ctgcaatttg tgtatgaata acagaataat ttccctcttt tgtttcgcct
      421 ttcctgttcc tgaatctaaa taaagatggc tttttagtat taaaagtgga agaaaattac
      481 aggtaattat ctttgacggt aaaaacgctg taatcagcgg gctacatgaa aaattactct
      541 aattatggct gcatttaaga gaatggaaaa aaaccttctt gtggataaaa accttaaatt
      601 gtccccaatg tctgcttcaa attggatggc actgcagctg gaggctttgt tcagaattga
      661 tcctggggag ctacgaaccc aaagtttcac agtaggaagc ttgatatcga attcctgcag
      721 cccgggctgg gcataaaagt cagggcagag ccatctattg cttacatttg cttctagcct
      781 gcaggtcgag gagcgcagcc ttccagaagc agagcgcggc gccttaagct gcagaagttg
      841 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
      901 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
      961 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
     1021 ttaagaaagc tagcgccacc atgtacccat acgatgttcc agattacgct accttgcaca
     1081 ataacagtac aacctcgcct ttgtttccaa acatcagctc ctcctggata cacagcccct
     1141 ccgatgcagg gctgcccccg ggaaccgtca ctcatttcgg cagctacaat gtttctcgag
     1201 cagctggcaa tttctcctct ccagacggta ccaccgatga ccctctggga ggtcataccg
     1261 tctggcaagt ggtcttcatc gctttcttaa cgggcatcct ggccttggtg accatcatcg
     1321 gcaacatcct ggtaattgtg tcatttaagg tcaacaagca gctgaagacg gtcaacaact
     1381 acttcctctt aagcctggcc tgtgccgatc tgattatcgg ggtcatttca atgaatctgt
     1441 ttacgaccta catcatcatg aatcgatggg ccttagggaa cttggcctgt gacctctggc
     1501 ttgccattga ctgcgtagcc agcaatgcct ctgttatgaa tcttctggtc atcagctttg
     1561 acagatactt ttccatcacg aggccgctca cgtaccgagc caaacgaaca acaaagagag
     1621 ccggtgtgat gatcggtctg gcttgggtca tctcctttgt cctttgggct cctgccatct
     1681 tgttctggca atactttgtt ggaaagagaa ctgtgcctcc gggagagtgc ttcattcagt
     1741 tcctcagtga gcccaccatt acttttggca cagccatcgc tggtttttat atgcctgtca
     1801 ccattatgac tattttatac tggaggatct ataaggaaac tgaaaagcgt accaaagagc
     1861 ttgctggcct gcaagcctct gggacagagg cagagacaga aaactttgtc caccccacgg
     1921 gcagttctcg aagctgcagc agttacgaac ttcaacagca aagcatgaaa cgctccaaca
     1981 ggaggaagta tggccgctgc cacttctggt tcacaaccaa gagctggaaa cccagctccg
     2041 agcagatgga ccaagaccac agcagcagtg acagttggaa caacaatgat gctgctgcct
     2101 ccctggagaa ctccgcctcc tccgacgagg aggacattgg ctccgagacg agagccatct
     2161 actccatcgt gctcaagctt ccgggtcaca gcaccatcct caactccacc aagttaccct
     2221 catcggacaa cctgcaggtg cctgaggagg agctggggat ggtggacttg gagaggaaag
     2281 ccgacaagct gcaggcccag aagagcgtgg acgatggagg cagttttcca aaaagcttct
     2341 ccaagcttcc catccagcta gagtcagccg tggacacagc taagacttct gacgtcaact
     2401 cctcagtggg taagagcacg gccactctac ctctgtcctt caaggaagcc actctggcca
     2461 agaggtttgc tctgaagacc agaagtcaga tcactaagcg gaaaaggatg tccctggtca
     2521 aggagaagaa agcggcccag accctcagtg cgatcttgct tgccttcatc atcacttgga
     2581 ccccatacaa catcatggtt ctggtgaaca ccttttgtga cagctgcata cccaaaacct
     2641 tttggaatct gggctactgg ctgtgctaca tcaacagcac cgtgaacccc gtgtgctatg
     2701 ctctgtgcaa caaaacattc agaaccactt tcaagatgct gctgctgtgc cagtgtgaca
     2761 aaaaaaagag gcgcaagcag cagtaccagc agagacagtc ggtcattttt cacaagcgcg
     2821 cacccgagca ggccttgtcc ggagataacg gaagcggagc cactaacttc tccctgttga
     2881 aacaagcagg ggatgtcgaa gagaatcccg ggccatctag aatggccccc aagaagaaga
     2941 ggaaggtggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca
     3001 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3061 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     3121 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
     3181 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     3241 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     3301 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     3361 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     3421 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     3481 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
     3541 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     3601 agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagtaa gtcgacggcg
     3661 cgccccaccc ctgcagggaa ttcgatatca agcttatcga taatcaacct ctggattaca
     3721 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3781 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3841 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3901 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3961 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4021 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4081 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga
     4141 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4201 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4261 gtcggatctc cctttgggcc gcctccccgc atcgataccg agcgctgctc gagagatctg
     4321 tgatagcggc catcaagctg gccgcgactc tagatcataa tcagccatac cacatttgta
     4381 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     4441 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4501 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4561 aaactcatca atgtatcagc ttatcgatac cgcatgcacg tgcggaccga gcggccgcag
     4621 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4681 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4741 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4801 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     4861 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4921 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4981 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5041 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5101 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5161 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5221 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     5281 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5341 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5401 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5461 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5521 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5581 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5641 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5701 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5761 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5821 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5881 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5941 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6001 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6061 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6121 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6181 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6241 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6301 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6361 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6421 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6481 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6541 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6601 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6661 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6721 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6781 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6841 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6901 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6961 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7021 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7081 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7141 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7201 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7261 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7321 ggttcctggc cttttgctgg ccttttgctc acatgt
//
