LOCUS       sequence-160325-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR reporter construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-atg4.2
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(430..454)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(573..593)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(601..618)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(601..617)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 actatacaat gcccggggga tcggtggagc tccaccggtg gcggccgctc cagaactagt
       61 ccaccaaagc catctgccaa gggagccaag aaggccgcca agaccgtcgt tgccaagcca
      121 aaggacggaa agaagagacg tcatgcccgc aaggaatcgt actccgtcta catctaccgt
      181 gttctcaagc aagttcaccc agacaccgga gtctcctcca aggccatgtc tatcatgaac
      241 tccttcgtca acgatgtatt cgaacgcatc gcttcggaag cttcccgtct tgctcattac
      301 aacaaacgct caacgatctc atcccgcgaa attcaaaccg ctgtccgttt gattctccca
      361 ggagaacttg ccaagcacgc cgtgtctgag ggaaccaagg ccgtcaccaa gtacacttcc
      421 agcaagtaaa cccagctttc ttgtacaaag tggttattga caatttttga aattctgtac
      481 actgaacatt tattggtgct tgatatgtat tgatgacgca atgtgaacat tattaaataa
      541 aaatgtatta agggaagaat tccatgtaac ttcaacttta ttatacatag ttgataattc
      601 actggccgtc gttttacact cgagacgtac ggtgcgcgcg atgcattcga agatctgccc
      661 actagtgagt cgtattataa gtgcaagtaa gatcagtgtt tgtttcgata tcagtctgtt
      721 tcgtaacggt cttctgtata actacaaaaa agaataaaaa accgtatcac aactgagaaa
      781 aaagacaaaa cttattgaac agtgttcctt tcaaaaaatt attttgtaaa aaatcgagtt
      841 tttctccaaa ataacgcacg caatcaaaaa caagaaaact aacgtgttct cctgggcgtg
      901 gaactcctta tcaagatac
//
