LOCUS       sequence-160326-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR reporter construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-atg4.2
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(608..628)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aagttgagtt catggaattc ttcccttaat acatttttat ttaataatgt tcacattgcg
       61 tcatcaatac atatcaagca ccaataaatg ttcagtgtac agaatttcaa aaattgtcaa
      121 taaccacttt gtacaagaaa gctgggttta cttgctggaa gtgtacttgg tgacggcctt
      181 ggttccctca gacacggcgt gcttggcaag ttctcctggg agaatcaaac ggacagcggt
      241 ttgaatttcg cgggatgaga tcgttgagcg tttgttgtaa tgagcaagac gggaagcttc
      301 cgaagcgatg cgttcgaata catcgttgac gaaggagttc atgatagaca tggccttgga
      361 ggagactccg gtgtctgggt gaacttgctt gagaacacgg tagatgtaga cggagtacga
      421 ttccttgcgg gcatgacgtc tcttctttcc gtcctttggc ttggcaacga cggtcttggc
      481 ggccttcttg gctcccttgg cagatggctt tggtggacta gttctggagc ggccgccacc
      541 ggtggagctc caccgatccc ccgggcattt gtatagttca tccatgccat gtgtaatccc
      601 agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga
      661 aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
      721 tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct
      781 aattttgaag ttctgaaaat ttaaatcaga ttagttagtt agtaaaatca tgtttaaact
      841 tacaactttg attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata
      901 gttgtattcc aatttgtgtc caagaatgtt tccatcttc
//
