LOCUS       sequence-160327-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing RHOA , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RHOA_WT
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             90..668
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     protein_bind    complement(670..769)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(786..805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(787..805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(810..826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(810..826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca tggctgccat ccggaagaaa ctggtgattg
      121 ttggtgatgg agcctgtgga aagacatgct tgctcatagt cttcagcaag gaccagttcc
      181 cagaggtgta tgtgcccaca gtgtttgaga actatgtggc agatatcgag gtggatggaa
      241 agcaggtaga gttggctttg tgggacacag ctgggcagga agattatgat cgcctgaggc
      301 ccctctccta cccagatacc gatgttatac tgatgtgttt ttccatcgac agccctgata
      361 gtttagaaaa catcccagaa aagtggaccc cagaagtcaa gcatttctgt cccaacgtgc
      421 ccatcatcct ggttgggaat aagaaggatc ttcggaatga tgagcacaca aggcgggagc
      481 tagccaagat gaagcaggag ccggtgaaac ctgaagaagg cagagatatg gcaaacagga
      541 ttggcgcttt tgggtacatg gagtgttcag caaagaccaa agatggagtg agagaggttt
      601 ttgaaatggc tacgagagct gctctgcaag ctagacgtgg gaagaaaaaa tctgggtgcc
      661 ttgtcttgtg cccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
      721 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
      781 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
      841 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
      901 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
      961 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     1021 tg
//
