LOCUS       sequence-160329-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing CDK4 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CDK4_WT
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca tggctacctc tcgatatgag ccagtggctg
      121 aaattggtgt cggtgcctat gggacagtgt acaaggcccg tgatccccac agtggccact
      181 ttgtggccct caagagtgtg agagtcccca atggaggagg aggtggagga ggccttccca
      241 tcagcacagt tcgtgaggtg gctttactga ggcgactgga ggcttttgag catcccaatg
      301 ttgtccggct gatggacgtc tgtgccacat cccgaactga ccgggagatc aaggtaaccc
      361 tggtgtttga gcatgtagac caggacctaa ggacatatct ggacaaggca cccccaccag
      421 gcttgccagc cgaaacgatc aaggatctga tgcgccagtt tctaagaggc ctagatttcc
      481 ttcatgccaa ttgcatcgtt caccgagatc tgaagccaga gaacattctg gtgacaagtg
      541 gtggaacagt caagctggct gactttggcc tggccagaat ctacagctac cagatggcac
      601 ttacacccgt ggttgttaca ctctggtacc gagctcccga agttcttctg cagtccacat
      661 atgcaacacc tgtggacatg tggagtgttg gctgtatctt tgcagagatg tttcgtcgaa
      721 agcctctctt ctgtggaaac tctgaagccg accagttggg caaaatcttt gacctgattg
      781 ggctgcctcc agaggatgac tggcctcgag atgtatccct gccccgtgga gcctttcccc
      841 ccagagggcc ccgcccagtg cagtcggtgg tacctgagat ggaggagtcg ggagcacagc
      901 tgctgctgga aatgctgact tttaacccac acaagcgaat ctctgccttt cgagctctgc
      961 agcactctta tctacataag gatgaaggta atccgg
//
