LOCUS       sequence-160332-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing EZH2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_EZH2_WT
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca tgggccagac tgggaagaaa tctgagaagg
      121 gaccagtttg ttggcggaag cgtgtaaaat cagagtacat gcgactgaga cagctcaaga
      181 ggttcagacg agctgatgaa gtaaagagta tgtttagttc caatcgtcag aaaattttgg
      241 aaagaacgga aatcttaaac caagaatgga aacagcgaag gatacagcct gtgcacatcc
      301 tgacttctgt gagctcattg cgcgggacta gggagtgttc ggtgaccagt gacttggatt
      361 ttccaacaca agtcatccca ttaaagactc tgaatgcagt tgcttcagta cccataatgt
      421 attcttggtc tcccctacag cagaatttta tggtggaaga tgaaactgtt ttacataaca
      481 ttccttatat gggagatgaa gttttagatc aggatggtac tttcattgaa gaactaataa
      541 aaaattatga tgggaaagta cacggggata gagaatgtgg gtttataaat gatgaaattt
      601 ttgtggagtt ggtgaatgcc cttggtcaat ataatgatga tgacgatgat gatgatggag
      661 acgatcctga agaaagagaa gaaaagcaga aagatctgga ggatcaccga gatgataaag
      721 aaagccgccc acctcggaaa tttccttctg ataaaatttt tgaagccatt tcctcaatgt
      781 ttccagataa gggcacagca gaagaactaa aggaaaaata taaagaactc accgaacagc
      841 agctcccagg cgcacttcct cctgaatgta cccccaacat agatggacca aatgctaaat
      901 ctgttcagag agagcaaagc ttacactcct ttcatacgct tttctgtagg cgatgtttta
      961 aatatgactg cttcctacat cgtaagtgca attattc
//
