LOCUS       sequence-160336-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            eCFP (no promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB443
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..233)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(244..246,247..249,250..762)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(289..310)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(550..569)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
      121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      181 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
      241 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      301 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      361 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      421 accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg
      481 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      541 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      601 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      661 cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag
      721 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      781 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      841 cactacctga gcacccagtc cgccctga
//
