LOCUS       sequence-160380-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing RAC1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RAC1_WT
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             85..660
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     protein_bind    complement(662..761)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(778..797)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(779..797)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(802..818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(802..818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
       61 ccaactttgt acaaaaaagt tggcatgcag gccatcaagt gtgtggtggt gggagacgga
      121 gctgtaggta aaacttgcct actgatcagt tacacaacca atgcatttcc tggagaatat
      181 atccctactg tctttgacaa ttattctgcc aatgttatgg tagatggaaa accggtgaat
      241 ctgggcttat gggatacagc tggacaagaa gattatgaca gattacgccc cctatcctat
      301 ccgcaaacag atgtgttctt aatttgcttt tcccttgtga gtcctgcatc atttgaaaat
      361 gtccgtgcaa agtggtatcc tgaggtgcgg caccactgtc ccaacactcc catcatccta
      421 gtgggaacta aacttgatct tagggatgat aaagacacga tcgagaaact gaaggagaag
      481 aagctgattc ccatcaccta tccgcagggt ctagccatgg ctaaggagat tggtgctgta
      541 aaatacctgg agtgctcggc gctcacacag cgaggcctca agacagtgtt tgacgaagcg
      601 atccgagcag tcctctgccc gcctcccgtg aagaagagga agagaaaatg cctgctgttg
      661 tacccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
      721 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
      781 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
      841 aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc
      901 caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg
      961 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatg
//
