LOCUS       sequence-160385-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP with 3 UAG codons at the amino acid position 3, 151, 
            and 153.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Schwessinger B, Li X, Ellinghaus TL, Chan LJ, Wei T, Joe A, Thomas 
            N, Pruitt R, Adams PD, Chern MS, Petzold CJ, Liu CC, Ronald PC
  TITLE     A second-generation expression system for tyrosine-sulfated proteins
            and its application in crop protection.
  JOURNAL   Integr Biol (Camb). 2016 Apr 18;8(4):542-5. doi: 10.1039/c5ib00232j.
            Epub 2015 Nov 27.
  PUBMED    26611838
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c5ib00232j"; journalName: "Integr Biol (Camb)"; date: 
            "2016-04-18- 18"; volume: "8"; issue: "4"; pages: "542-5"
COMMENT     Sequence Label: pGLO-GFP-3UAG
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..759
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH*V*ITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(73..101)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     696..716
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    761..817
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgg cttagagcaa aggagaagaa
       61 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      121 ttttctgtca gtggagaggg tgaaggtgat gctacatacg gaaagcttac ccttaaattt
      181 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttctcttat
      241 ggtgttcaat gcttttcccg ttatccggat catatgaaac ggcatgactt tttcaagagt
      301 gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga cgggaactac
      361 aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat cgagttaaaa
      421 ggtattgatt ttaaagaaga tggaaacatt ctcggacaca aactcgagta caactataac
      481 tcacactagg tatagatcac ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa
      541 attcgccaca acattgaaga tggatccgtt caactagcag accattatca acaaaatact
      601 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtcgac acaatctgcc
      661 ctttcgaaag atcccaacga aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct
      721 gctgggatta cacatggcat ggatgagctc tacaaataat gaattcgagc tcggtacccg
      781 gggatcctct agagtcgacc tgcaggcatg caagcttggc tgttttggcg gatgagagaa
      841 gattt
//
