LOCUS       sequence-160387-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP with 1 UAG codon at the amino acid position 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Schwessinger B, Li X, Ellinghaus TL, Chan LJ, Wei T, Joe A, Thomas 
            N, Pruitt R, Adams PD, Chern MS, Petzold CJ, Liu CC, Ronald PC
  TITLE     A second-generation expression system for tyrosine-sulfated proteins
            and its application in crop protection.
  JOURNAL   Integr Biol (Camb). 2016 Apr 18;8(4):542-5. doi: 10.1039/c5ib00232j.
            Epub 2015 Nov 27.
  PUBMED    26611838
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c5ib00232j"; journalName: "Integr Biol (Camb)"; date: 
            "2016-04-18- 18"; volume: "8"; issue: "4"; pages: "542-5"
COMMENT     Sequence Label: pGLO-GFP-1UAG
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..755
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(69..97)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     692..712
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    757..813
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatggctta gagcaaagga gaagaacttt
       61 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      121 ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa gcttaccctt aaatttattt
      181 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc tcttatggtg
      241 ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc aagagtgcca
      301 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg aactacaaga
      361 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta
      421 ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac tataactcac
      481 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
      541 gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa aatactccaa
      601 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgcccttt
      661 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
      721 ggattacaca tggcatggat gagctctaca aataatgaat tcgagctcgg tacccgggga
      781 tcctctagag tcgacctgca ggcatgcaag cttggctgtt ttggcggatg agagaagatt
      841 t
//
