LOCUS       sequence-160388-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing JAK2 , part of the Target 
            Accelerator Plasmid Collection. *Please note this is not a true WT 
            JAK2 sequence and contains a V617F mutation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_JAK2_WT
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt
       61 tggcaccatg ggaatggcct gccttacgat gacagaaatg gagggaacat ccacctcttc
      121 tatatatcag aatggtgata tttctggaaa tgccaattct atgaagcaaa tagatccagt
      181 tcttcaggtg tatctttacc attcccttgg gaaatctgag gcagattatc tgacctttcc
      241 atctggggag tatgttgcag aagaaatctg tattgctgct tctaaagctt gtggtatcac
      301 acctgtgtat cataatatgt ttgctttaat gagtgaaaca gaaaggatct ggtatccacc
      361 caaccatgtc ttccatatag atgagtcaac caggcataat gtactctaca gaataagatt
      421 ttactttcct cgttggtatt gcagtggcag caacagagcc tatcggcatg gaatatctcg
      481 aggtgctgaa gctcctcttc ttgatgactt tgtcatgtct tacctctttg ctcagtggcg
      541 gcatgatttt gtgcacggat ggataaaagt acctgtgact catgaaacac aggaagaatg
      601 tcttgggatg gcagtgttag atatgatgag aatagccaaa gaaaacgatc aaaccccact
      661 ggccatctat aactctatca gctacaagac attcttacca aaatgtattc gagcaaagat
      721 ccaagactat catattttga caaggaagcg
//
