LOCUS       sequence-160393-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PPP2R1A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PPP2R1A_WT
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgacaac tttgtacaaa aaagttggca ccatggcggc ggccgacggc gacgactcgc
      121 tgtaccccat cgcggtgctc atagacgaac tccgcaatga ggacgttcag cttcgcctca
      181 acagcatcaa gaagctgtcc accatcgcct tggcccttgg ggttgaaagg acccgaagtg
      241 agcttctgcc tttccttaca gataccatct atgatgaaga tgaggtcctc ctggccctgg
      301 cagaacagct gggaaccttc actaccctgg tgggaggccc agagtacgtg cactgcctgc
      361 tgccaccgct ggagtcgctg gccacagtgg aggagacagt ggtgcgggac aaggcagtgg
      421 agtccttacg ggccatctca cacgagcact cgccctctga cctggaggcg cactttgtgc
      481 cgctagtgaa gcggctggcg ggcggcgact ggttcacctc ccgcacctcg gcctgcggcc
      541 tcttctccgt ctgctacccc cgagtgtcca gtgctgtgaa ggcggaactt cgacagtact
      601 tccggaacct gtgctcagat gacaccccca tggtgcggcg ggccgcagcc tccaagctgg
      661 gggagtttgc caaggtgctg gagctggaca acgtcaagag tgagatcatc cccatgttct
      721 ccaacctggc ctctgacgag caggactcgg tgcggctgct ggcggtggag gcgtgcgtga
      781 acatcgccca gcttctgccc caggaggatc tggaggccct ggtgatgccc actctgcgcc
      841 aggccgctga agacaagtcc tggcgcgtcc gctacatggt ggctgacaag ttcacagagc
      901 tccagaaagc agtggggcct gagatcacca agacagacct ggtccctgcc ttccagaacc
      961 tgatgaaaga ctgtgaggcc gaggtgaggg ccgcagcctc ccacaaggtc aa
//
