LOCUS       sequence-160396-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of mRuby3 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:20889. doi"; pages: " 
            10.1038/srep20889"
COMMENT     Sequence Label: pNCS-mRuby3
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             48..70
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             111..143
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(147..155,156..170)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             177..887
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
ORIGIN
        1 tattcttgac acctgatgcg atgaatgata taataggaaa gtactgtttt gtttaacttt
       61 aagaaggaga tatacatatg cggggttctc atcatcatca tcatcatggt atggctagca
      121 tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatccgatgg
      181 tgtctaaggg cgaagagctg atcaaggaaa atatgcgtat gaaggtggtc atggaaggtt
      241 cggtcaacgg ccaccaattc aaatgcacag gtgaaggaga aggcagaccg tacgagggaa
      301 ctcaaaccat gaggatcaaa gtcatcgagg gaggacccct gccatttgcc tttgacattc
      361 ttgccacgtc gttcatgtat ggcagccgta cttttatcaa gtacccggcc gacatccctg
      421 atttctttaa acagtccttt cctgagggtt ttacttggga aagagttacg agatacgaag
      481 atggtggagt cgtcaccgtc acgcaggaca ccagccttga ggatggcgag ctcgtctaca
      541 acgtcaaggt cagaggggta aactttccct ccaatggtcc cgtgatgcag aagaagacca
      601 agggttggga gcctaataca gagatgatgt atccagcaga tggtggtctg agaggataca
      661 ctgacatcgc actgaaagtt gatggtggtg gccatctgca ctgcaacttc gtgacaactt
      721 acaggtcaaa aaagaccgtc gggaacatca agatgcccgg tgtccatgcc gttgatcacc
      781 gcctggaaag gatcgaggag agtgacaatg aaacctacgt agtgcaacgc gaagtggcag
      841 ttgccaaata cagcaacctt ggtggtggca tggacgagct gtacaagtaa tgaattcgaa
      901 gcttgatccg gctgctaaca a
//
