LOCUS       sequence-160397-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of mRuby3 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:20889. doi"; pages: " 
            10.1038/srep20889"
COMMENT     Sequence Label: pNCS-mRuby3
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..741)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     CDS             complement(join(748..762,763..771))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(775..807)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(811..828)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atcaagcttc gaattcatta cttgtacagc tcgtccatgc caccaccaag
       61 gttgctgtat ttggcaactg ccacttcgcg ttgcactacg taggtttcat tgtcactctc
      121 ctcgatcctt tccaggcggt gatcaacggc atggacaccg ggcatcttga tgttcccgac
      181 ggtctttttt gacctgtaag ttgtcacgaa gttgcagtgc agatggccac caccatcaac
      241 tttcagtgcg atgtcagtgt atcctctcag accaccatct gctggataca tcatctctgt
      301 attaggctcc caacccttgg tcttcttctg catcacggga ccattggagg gaaagtttac
      361 ccctctgacc ttgacgttgt agacgagctc gccatcctca aggctggtgt cctgcgtgac
      421 ggtgacgact ccaccatctt cgtatctcgt aactctttcc caagtaaaac cctcaggaaa
      481 ggactgttta aagaaatcag ggatgtcggc cgggtacttg ataaaagtac ggctgccata
      541 catgaacgac gtggcaagaa tgtcaaaggc aaatggcagg ggtcctccct cgatgacttt
      601 gatcctcatg gtttgagttc cctcgtacgg tctgccttct ccttcacctg tgcatttgaa
      661 ttggtggccg ttgaccgaac cttccatgac caccttcata cgcatatttt ccttgatcag
      721 ctcttcgccc ttagacacca tcggatcctt atcgtcatcg tcgtacagat cccgacccat
      781 ttgctgtcca ccagtcatgc tagccatacc atgatgatga tgatgatgag aaccccgcat
      841 atgtatatct ccttcttaaa gtta
//
