LOCUS       sequence-160399-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing AKT1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_AKT1_WT
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca ccatgagcga cgtggctatt gtgaaggagg
      121 gttggctgca caaacgaggg gagtacatca agacctggcg gccacgctac ttcctcctca
      181 agaatgatgg caccttcatt ggctacaagg agcggccgca ggatgtggac caacgtgagg
      241 ctcccctcaa caacttctct gtggcgcagt gccagctgat gaagacggag cggccccggc
      301 ccaacacctt catcatccgc tgcctgcagt ggaccactgt catcgaacgc accttccatg
      361 tggagactcc tgaggagcgg gaggagtgga caaccgccat ccagactgtg gctgacggcc
      421 tcaagaagca ggaggaggag gagatggact tccggtcggg ctcacccagt gacaactcag
      481 gggctgaaga gatggaggtg tccctggcca agcccaagca ccgcgtgacc atgaacgagt
      541 ttgagtacct gaagctgctg ggcaagggca ctttcggcaa ggtgatcctg gtgaaggaga
      601 aggccacagg ccgctactac gccatgaaga tcctcaagaa ggaagtcatc gtggccaagg
      661 acgaggtggc ccacacactc accgagaacc gcgtcctgca gaactccagg caccccttcc
      721 tcacagccct gaagtactct ttccagaccc acgaccgcct ctgctttgtc atggagtacg
      781 ccaacggggg cgagctgttc ttccacctgt cccgggagcg tgtgttctcc gaggaccggg
      841 cccgcttcta tggcgctgag attgtgtcag ccctggacta cctgcactcg gagaagaacg
      901 tggtgtaccg ggacctcaag ctggagaacc tcatgctgga caaggacggg cacattaaga
      961 tcacagactt cgggctgtgc aaggagggga tcaaggacgg tgccaccatg aagacctttt
     1021 gc
//
