LOCUS       sequence-160421-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu
            K, Bokoch GM, Hahn KM
  TITLE     Rho family proteins modulate rapid apoptosis induced by cytotoxic T 
            lymphocytes and Fas.
  JOURNAL   J Biol Chem. 2000 Mar 31. 275(13):9725-33.
  PUBMED    10734125
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Mar 31. 275(13):9725-33."
COMMENT     Sequence Label: pcDNA3-EGFP-Rac1-wt
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..601
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     promoter        complement(629..647)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(630..647)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(667..684)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    673..784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggacgagctg tacaaggaat tcatgcaggc catcaagtgt gtggtggtgg gagacggagc
       61 tgtaggtaaa acttgcctac tgatcagtta cacaaccaat gcatttcctg gagaatatat
      121 ccctactgtc tttgacaatt attctgccaa tgttatggta gatggaaaac cggtgaatct
      181 gggcttatgg gatacagctg gacaagaaga ttatgacaga ttacgccccc tatcctatcc
      241 gcaaacagat gtgttcttaa tttgcttttc ccttgtgagt cctgcatcat ttgaaaatgt
      301 ccgtgcaaag tggtatcctg aggtgcggca ccactgtccc aacactccca tcatcctagt
      361 gggaactaaa cttgatctta gggatgataa agacacgatc gagaaactga aggagaagaa
      421 gctgactccc atcacctatc cgcagggtct agccatggct aaggagattg gtgctgtaaa
      481 atacctggag tgctcggcgc tcacacagcg aggcctcaag acagtgtttg acgaagcgat
      541 ccgagcagtc ctctgcccgc ctcccgtgaa gaagaggaag agaaaatgcc tgctgttgta
      601 actcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct
      661 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
      721 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
      781 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
      841 agggggagga t
//
