LOCUS       sequence-160422-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-responsive fluorescent reporter plasmid containing a 
            STOP-cassette flanked by loxP sites followed by ZsGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-loxPSTOPloxP-ZsGreen
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    87..120
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    287..421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    541..675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gttcatgcct tcttcttttt cctacagatc cttaattaat aatacgactc actatagggg
       61 gtcgacccgc caccaatcat ttaaatataa cttcgtataa tgtatgctat acgaagttat
      121 tcgcgatgaa taaatgaaag cttgcagatc tgcgactcta gaggatctgc gactctagag
      181 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      241 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      301 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      361 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
      421 ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
      481 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
      541 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
      601 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
      661 tatcatgtct ggatctgcga ctctagagga tcataatcag ccataccaca tttgtagagg
      721 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      781 caattgttgt tgttaacttg tttattgcag cttataatgg ttac
//
