LOCUS       sequence-160441-        6745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid co-expressed with VSVG allows the production of mCAT-1 
            gesicles rendering target cells permissive to transduction with 
            Ecotropic lenti/retrovectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Mangeot PE, Dollet S, Girard M, Ciancia C, Joly S, Peschanski M, 
            Lotteau V
  TITLE     Protein transfer into human cells by VSV-G-induced nanovesicles.
  JOURNAL   Mol Ther. 2011 Sep;19(9):1656-66. doi: 10.1038/mt.2011.138. Epub 
            2011 Jul 12.
  PUBMED    21750535
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2011.138"; journalName: "Mol Ther"; date: "2011-09"; 
            volume: "19"; issue: "9"; pages: "1656-66"
COMMENT     Sequence Label: pp-mCAT1
FEATURES             Location/Qualifiers
     source          1..6745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1365
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1373..1392
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3449..3468)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3514..3569
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3568..3587)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3927..3943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3927..3943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3940..3962)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3951..3967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3975..3981,3982..3999,4000..4005))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4020..4041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4158..4175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4329..4917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4409..4428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5386..5765)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     5712..5731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5950..6054)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6080..6535)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6217..6238)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6429..6448
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6662..6684
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6676..6693
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6677..6693
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6703..6722
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6703..6721
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt cttgtaactt tttcgttaaa ctttagcttg
     1021 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc
     1081 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag
     1141 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg
     1201 cgtggaaata ttcttattgg tagaaacaac tacatcctgg tcatcatcct gcctttctct
     1261 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg
     1321 ggcccctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     1381 ctggttattg tgctgtctca tcattttggc aaagaattcc tcgagggcgc gccatgcatt
     1441 gatcaagctt gatatcctgc agacgcgtag gatctaccat gggctgcaaa aacctgctcg
     1501 gtctgggcca gcagatgctg cgccggaagg tggtggactg cagccgggag gagagccggc
     1561 tgtcccgctg cctcaacacc tatgacctgg tagctcttgg ggtgggcagc accttgggcg
     1621 ctggtgtcta tgtcctagcc ggtgccgtgg cccgtgaaaa tgctggccct gccatcgtca
     1681 tctccttctt gattgctgct ctcgcctccg tgctggccgg cctgtgctac ggcgagtttg
     1741 gtgcccgtgt ccccaagacg ggctcagcct acctctacag ctacgtgacg gtgggggagc
     1801 tttgggcctt catcactggc tggaacctga ttctctccta catcatcggt acttcaagcg
     1861 tggcaagagc ctggagtgcg acttttgacg agctgatagg caagcccatc ggagagttct
     1921 cacgtcagca catggccctg aatgctcctg gggtgctggc ccaaaccccg gacatatttg
     1981 ctgtgattat aattatcatc ttaacaggac tgttaactct tggcgtgaag gagtcagcca
     2041 tggtcaacaa aattttcacc tgtatcaatg tcctggtctt gtgcttcatc gtggtgtccg
     2101 ggttcgtgaa aggctccatt aaaaactggc agctcacgga gaaaaatttc tcctgtaaca
     2161 acaacgacac aaacgtgaaa tacggtgagg gagggtttat gccctttgga ttctctggtg
     2221 tcctgtcagg ggcagcgacc tgcttttatg ccttcgtggg ctttgactgc atcgccacca
     2281 caggggaaga agtcaagaac ccccagaagg ccattcctgt gggcatcgtg gcgtccctcc
     2341 tcatttgctt catagcgtac tttggcgtgt ccgccgctct cacgctcatg atgccttact
     2401 tctgcctgga catcgacagc ccgctgcctg gtgccttcaa gcaccagggc tgggaagaag
     2461 ctaagtacgc agtggccatt ggctctctct gcgcactttc caccagtctc ctaggctcca
     2521 tgtttcccat gccccgagtt atctatgcca tggctgaaga tggactactg tttaaatttt
     2581 tggccaaaat caacaatagg accaaaacac ccgtaatcgc cactgtgacc tcaggcgcca
     2641 ttgctgctgt gatggccttc ctctttgaac tgaaggacct ggtggacctc atgtccattg
     2701 gcactctcct ggcttactct ttggtggctg cctgtgtttt ggtcttacgg taccagccag
     2761 aacaacctaa tctggtatac cagatggcca gaaccaccga ggagctagat cgagtagatc
     2821 agaatgagct ggtcagtgcc agtgaatcac agacaggctt tttaccggta gccgagaagt
     2881 tttctctgaa atccatcctc tcacccaaga acgtggagcc ctccaaattc tcagggctaa
     2941 ttgtgaacat ttcagccggc ctcctagccg ctcttatcat caccgtgtgc attgtggccg
     3001 tgcttggaag agaggccctg gccgaaggga cactgtgggc agtctttgta atgacagggt
     3061 cagtcctcct ctgcatgctg gtgacaggca tcatctggag acagcctgag agcaagacca
     3121 agctctcatt taaggtaccc tttgtccccg tacttcctgt cttgagcatc ttcgtgaaca
     3181 tctatctcat gatgcagctg gaccagggca cgtgggtccg gtttgcagtg tggatgctga
     3241 taggtttcac catctatttc ggttatggga tctggcacag tgaggaagcg tccctggctg
     3301 ctggccaggc aaagactcct gacagcaact tggaccagtg caaatgaaag cttggatccg
     3361 tcgaggaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
     3421 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
     3481 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     3541 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     3601 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     3661 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     3721 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     3781 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     3841 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     3901 acggatcggc cgcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     3961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4021 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     4081 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4141 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4741 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggg ttagctcctt
     5521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5701 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5761 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5821 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5881 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5941 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6001 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6061 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     6121 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     6181 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     6241 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     6301 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     6361 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     6421 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     6481 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     6541 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     6601 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     6661 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     6721 ggcgaattgg agctccaccg cggtg
//
