LOCUS       sequence-160453-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc tag and mRNA capping enzyme without 25 amino acid from
            C-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Mukherjee C, Bakthavachalu B, Schoenberg DR
  TITLE     The cytoplasmic capping complex assembles on adapter protein nck1 
            bound to the proline-rich C-terminus of Mammalian capping enzyme.
  JOURNAL   PLoS Biol. 2014 Aug 19;12(8):e1001933. doi: 
            10.1371/journal.pbio.1001933. eCollection 2014 Aug.
  PUBMED    25137142
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2014-08-19"; volume: "12(8):e1001933. doi"; pages: " 
            10.1371/journal.pbio.1001933. eCollection 2014 Aug"
COMMENT     Sequence Label: pCDNA3-myc- mCE(Del 25C)
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttggtaccac catggcatca atgcagaagc tgatctcaga
      121 ggaggacctg cttatggcca tggaggccgc ttacaacaag atcccgccgc ggtggctgaa
      181 ctgtccgcgg cgcggtcagc cggtggcagg aagattctta cctctgaaga cgatgctggg
      241 gccaagatat gatagtcaag ttgctgaaga aaaccggttc catcccagta tgctctcaaa
      301 ttatctaaag agcctgaagg ttaaaatgag cttgttggtg gatctgacaa atacttcaag
      361 gttctatgac agaaatgaca tagaaaaaga aggaatcaag tatatcaaac ttcagtgtaa
      421 aggacatggc gaatgcccca ccactgagaa tactgagaca ttcattcgcc tgtgtgagag
      481 gtttaatgaa agaagcccac ctgaacttat aggtgttcac tgcacccatg gcttcaaccg
      541 cactggtttc cttatatgtg cctttttggt ggaaaaaatg gattggagta ttgaagcagc
      601 agttgctaca tttgcccaag ccagaccacc aggaatctac aagggtgatt atttgaagga
      661 actttttcgt cgctatggag atatagaaga agcaccacca ccacctgtat tgccagattg
      721 gtgttttgaa gatgaggatg aagaagatga ggatgaagat ggaaaaaagg actcagaacc
      781 agggtcaagt gcatccttta gtaaaaggag aaaagagcgg ttaaaactgg gagctatttt
      841 cttggaaggc ataactgtta aaggtgtaac tcaagtaaca actcaaccca agttaggaga
      901 ggtgcagcag aaatgtcatc agttctgtgg ctgggaaggg tctgggttcc ctggagca
//
